To answer these questions, the researchers applied live-cell imaging to the earliest phase of dissociation in human and mouse ES cells.
为了解答这些问题，研究人员运用活细胞成像技术到人胚胎干细胞和鼠胚胎干细胞最初的分离阶段。
2
Methods We used the improved enzymatic dissociation method and the patch clamp whole cell recording.
方法采用改进的酶解分离法分离单个细胞并经膜片钳全细胞记录法进行鉴定。
3
Mechanical dissociation was used to obtain single primary spermatocytes from guinea pigs and iron channel currents in the primary spermatocytes were recorded by whole cell patch-clamp technique.