The mutant G138P was obtained by in vitro sitedirectedmutagenesis of GI gene.
用双引物法对GI基因进行体外定点突变,构建了GI突变体g 138p。
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AIM: To establish the mutant of coding calcium binding fragment of the 13th exon of human thrombospondin-1 (TSP-1) gene with polymerase chain reaction (PCR) sitedirectedmutagenesis technology.
目的:利用聚合酶链反应定点突变技术构建人血小板反应素1基因第13外显子编码钙结合域突变体。
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Objective: To develop a simple and efficient way to perform site-directedmutagenesis.