Methods a retroviralvector containing TK gene was constructed and transduced into pulmonary adenocarcinoma cell A549 by electroporation.
方法构建含t K基因的逆转录病毒表达载体,电穿孔法转化肺癌细胞A549。
2
Adenovirus vector is an earlier and maturer genetic carrier in the respect of gene therapy and genetic immunization after retroviralvector.
腺病毒载体是继逆转录病毒载体后在基因治疗、基因免疫等方面应用开发得较早且较成熟的一种基因载体。
3
Conclusion Successfully constructed retroviralvector encoding for HPV16E7 establishes important basis for gene transfection of human articular chondrocytes.