Now when we put insulin in this place behind the right promoter, the cell thinks that it still has the lacZ gene present which it needs for its metabolism.

现在我们把胰岛素基因,放在正确的启动子后面,宿主细胞就会认为,它仍然有新陈代谢所需的lacZ基因

生物医学工程探索课程节选 : 耶鲁公开课

What we're going to do is take this plasmid, with its promoter gene construct in it and engineer it, to take out the lacZ gene, leaving in the promoter and putting in the protein that we want - the gene for the protein that we want.

我们要做的就是提取包含,它自己的启动子基因的质粒,并对其进行一些修改,剔除lacZ基因,留下启动子,把我们的目标蛋白植入--,我们生产目标蛋白的基因

生物医学工程探索课程节选 : 耶鲁公开课