Objective To examine the changes of conformation and activity of human placental alkaline phosphatase(HPAP) in denaturation and renaturation of guanidine chloride, and to study its folding mechanism.
目的观测人胎盘碱性磷酸酶（HPAP）在盐酸胍变性和复性过程中构象与活力的变化，探讨其折叠机制。
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The recombination fusion protein was purified by lysis, washing, denaturation and renaturation. The specific band of expression was excised from the gel and used to immunize mice .