Each individual is unique in the amount of energy they can hold and activate in their personal bio-system in the process of DNA-template activation.

The aerobic bio-treatment process with the activated sludge method is a complex system which has the properties of essential nonlinearity and strong disturbance to treat city sewage.

On basis of the advanced study of bio-regeneration process, it is thought that ectoenzyme hypothesis does not come into existence in this system.