A self cleaving ribozyme was comprised of a hammerhead ribozyme and its targetsequence located the downstream of the hammerhead ribozyme.
在锤头型核酶下游增加一段核酶作用的靶序列，使之成为自切割的核酶。
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This process is composed of such sections as recognition of target template, alignment between query sequence and targetsequence, modeling, evaluation of structure rationality.
Conclusion PNA combining with the DNA of targetsequence had a highly specificity. Choosing PNA as the probe may improve the determined specificity of piezoelectric gene sensor.