AIM: To report on an in vitro reconstruction of corneal stroma and analyze the consequences of collagen contraction in view of cell biology and engineering.
目的：利用细胞生物学和工程学原理在体外重建角膜基质结构，分析影响角膜胶原收缩的因素。
2
Corneal stroma cells formed triangle and arborization, cell-cell junction was obvious, and formed network link.
角膜基质细胞拉长生长，呈三角形或树枝状，细胞间连接明显，可形成网状连接。
3
Methods under the same condition with hematopoietic factors in the liquid culture, compared the sequential expansion group and once for all expansion group with or without stromacell layer.