However, amplification of GC-rich templates, as one of the bottlenecks in PCR, has not been solved thoroughly.
而高gc模板扩增作为P CR技术中的瓶颈问题之一，目前仍然没有得到较好的解决。
2
To ensure that your solution is capable of scaling and that there no bottlenecks on your new system, testing needs to be done properly.
为了确保你的解决方案可以适用于大范围，而且你的新系统没有瓶颈存在，那么需要正确的进行测试。
3
The tanker traffic jam outside Qingdao is one of several bottlenecks in the global oil sector that have pushed up tanker rates as vessels are held up in the queues.