Methods The whole intron 51 was sequenced by primerwalking.
方法用步移法测定第5 1内含子全序列。
2
Single primer PCR was a method of chromosome walking to isolate sequences flanking a known DNA sequence with only one primer.
单引物pcr是一种只用一条引物就可以克隆已知序列侧翼未知区域的染色体步移方法。
3
Conclusion It is a feasible method to discover and acquire novel genes of S. japonicum by ESTs, combination of primer-walking sequncing and bioinformatics technique.