Doing foreground selection of BC_1F_1 with polymorphiclocus, 161 donor segment were detected, and doing background selection and backcross for plants containing donor segment.
A total of 174 alleles were detected across the 23 microsatellite loci. All the loci were polymorphic and the number of alleles varied from 3 to 12, and the mean number was 7.5 alleles per locus.