Inclusion body proteinrenaturation is a important procedure in the biotechnology.
包涵体蛋白的复性是生物工程下游技术中的一个重要难题。
2
Fusion protein expression was induced by IPTG. After renaturation, the protein was purified by affinity chromatography and the bioactivity was examined by ELISA.
IPTG诱导表达融合蛋白,包涵体蛋白经复性后亲和层析法纯化,ELISA方法测定蛋白活性。
3
The process of renaturation is established in the understanding to the mechanism of protein folding in vitro.