Methods Spectrophotometry was used to determine the changes of activity of HPAP during guanidine denaturation in the presence and absence of phosphate.
方法应用分光光度法测定有磷酸盐及无磷酸盐存在时,HPAP在胍变性过程中的活力变化。
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Objective To study the effects of phosphate on guanidine denaturation of human placental alkaline phosphatase (HPAP) and provide a theoretical basis for flexibility hypothesis of enzyme active sites.