An improved nearest neighbor subtraction algorithm was presented and applied in the Computational Optical Section Microscopy (COSM).

Objective to develop a method for the optical section of laser scanning confocal microscopy of amnion and to study the spatial structure of cell and actin cytoskeleton in amnion of human embryo.

the use of optical vortex beams with a doughnut-like shape in the cross-section to form an optical vortex pipeline;