Methods: Based on the rat's hypertension model made by clipping both kidneys, we made a CCS model by infusing collagenase and heparin into the rat's caudate nucleus.

Silicon acts as a protective barrier to the skin that reduces trans-epidural water loss and helps to increase collagenase activity which reduces the hardening and raised appearance of scars.

This paper reports the successful isolation of islets of Langerhans from the rat pancreas using a modified collagenase digestion, Ficoll density gradient separation and hand-picking technique.