The binding reaction between 1-(2-pyridylazo)-2-naphthol-sulfonic acid(PAN-S) and bovine serum albumin(BSA) was studied by using fluorescence spectra and absorption spectra.
The color reaction conditions of Cu 2+ and 1-(2-pyridylazo)-2-naphthol(PAN)were researched, and a new method was developed for the determination of copper in sulfuric acid anodizing liquor.