Methods DEAE-Cellulose and gel-filtrationchromatography were used to isolate and purify the polysaccharides, whose structures were characterized by chemical and spectral methods.
方法采用DEAE -纤维素柱色谱和凝胶渗透色谱分离纯化，化学和光谱方法分析其结构特征。
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The purity of fusion protein was about 70% after ultrasonic decomposition and washing repeatedly, and reached above 95 % after purification by ion exchange and gel-filtrationchromatography.
The results showed that the methods of isoelectric precipitation, gel-filtrationchromatography and anion-exchange chromatography were suitable for purifying the major allergens in buffalo milk.