As hydrogen peroxide and freeradicalintermediate participate in enzyme deactivation, loss of catalytic activity of HRP in phenol oxidative polymerization can be greatly accelerated.
催化酚和过氧化物的氧化反应中,反应物和产物参与酶的失活过程,使失活速率增加;
2
METHODS The reaction product and freeradicalintermediate in the reaction medium with or without antioxidants and other moderators was detected by spectrophotometry and electron spin resonance(ESR).