随着它向细胞表面迁移 ，Gag和Gag-Pol多聚蛋白被病毒蛋白酶切割，形成一个成熟的能够感染的病毒颗粒 [...][2] [9] 。 labome.cn | As it migrates towards the cell [...] surface , the Gag and Gag-Polpolyproteins [...]are cleaved by the viral protease, resulting in [...]a mature, infectious particle [2] [9] . labome.com |
简单和复杂的逆转录病毒都含有两份线性、不分节段的、长7-12 kb的单链RNA，编 码gag,pol和env基因。 labome.cn | Simple and complex retroviruses both [...] contain two copies of linear, nonsegmented, single-stranded RNA of 7-12 kb in [...] length encoding thegag,pol, and envgenes. labome.com |
慢病毒载体生产的第一步是将转运质粒、包膜质粒 和gag-pol质粒与包装细胞共转染（如果是逆转录病毒载体生产则为单次转染转运质粒）。 labome.cn | The first step in lentiviral vector production is [...] co-transfection of the packaging cells with the [...] transfer, envelope, andgag-polplasmids (or single [...]transfection of the transfer plasmid [...]in the case of retroviral vector production). labome.com |
对逆转录病毒而言，转运载体被转染至Phoenix细胞内，Phoenix细胞是带有两个辅助构建体（e nv和gag-pol）作为附加体的人胚肾293T细胞。 labome.cn | For retroviruses, the transfer vector is transfected into Phoenix [...] cells, a cell line based on Human Embryonic Kidney 293T that carries the two helper [...] constru cts (env andgag-pol) as episomes. labome.com |
注意：由于γ-逆转录病毒和慢病 毒gag、pol和env基因间的亚型差异，转运质粒和包装质粒不能够互换。 labome.cn | Note: Due to isoform differences between [...] γ-retroviral and lentiviral gag, pol,andenv genes, [...]the packaging plasmids are not interchangeable with the transfer plasmids. labome.com |
当 Gag和Gag-Pol多聚蛋白被病毒蛋白酶切割后，病毒颗粒成熟。 labome.cn | The particle [...] matures wh entheGag andGag-Pol polyproteins [...]are cleaved by the viral protease. labome.com |
Gag和Pol：这些病毒蛋白是病毒粒子的成熟所需要的。 labome.cn | Gag and Pol: These viral [...]proteins are needed for maturation of the virion. labome.com |
逆转录病毒的单链RNA呈线性，不分节段，长7-12 kb，编 码gag、pol和env基因。 labome.cn | The linear, nonsegmented, single-stranded RNA of retroviruses is 7-12 kb in length [...] and enco dethegenesgag,pol, and env. labome.com |
王国兴刚才花了很多时间，说甚麽“一国两制”是好的，但一个街市 有两重租值却不好了 ⎯⎯ 他以为这样说 很gag，但我觉得这是 烂gag，而且街市内何止两重租值呢，“老兄”？ legco.gov.hk | Mr WONG Kwok-hing just now spent a lot of time praising "one country, two [...] systems" while condemning "one market, two rent levels" [...] ― he thought this isa good gag, but I think it is indeed lame. legco.gov.hk |