| 单词 | 转染 | ||||||||||||||||||||||||||||||||||||||||||||||||||||
| 释义 | 转染 noun —transfection nExamples:非转染 adj—non-transfected See also:染 n—contamination n • dye n 染—catch (a disease) • add color washes to a painting • acquire (bad habits etc)
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转染试剂可以通过商业途径获得或者在实验室中配制。 labome.cn | Transfetion reagents can be acquired commercially or formulated in the lab. labome.com |
本应用报告对潜在非病毒基因载体的表征进行了说明，显示了 其 转染 能 性，无显著的... malvern.com.cn | This application note presents the characterization of a potential nonviral gene carrier [...] that has shown transfection ability without [...]significant... malvern.com |
首先，可以用质粒载体转染细胞 ，该载体上携带荧光蛋白基因，以及将蛋白质同特定细胞器分离的足够肽段信息。 labome.cn | First, one can transfect cells with a [...] vector coding for a fluorescent protein and sufficient peptide information to cause [...]the protein to segregate to the specific organelle. labome.com |
用克隆形成细胞活存率实验和转染检 测PSP94和CRISP-3的异位整合表达对细胞生长的作用。 asiaandro.com | The effect that ectopic expression of PSP94 or CRISP-3 had on cell [...] growth was studied by clonogenic survival [...] assay following transfection. To evaluate the [...]effects of co-expression of the two [...]proteins, stable clones of PC3 that expressed PSP94 were generated. asiaandro.com |
除了最常用的转染试剂 （lipofectamine、fugene、磷酸钙等），还有很多商品化的试剂盒，含有转运和辅助质粒，有更高的病毒产量和优化了的质粒比。 labome.cn | In addition to [...] the most common transfection reagents that are [...]available (lipofectamine, fugene, calcium phosphate, etc), [...]there are also many commercially formulated kits that contain the transfer and helper plasmids and tout higher virus yield and optimized ratio of plasmids. labome.com |
它由专利配方的正电荷脂质体和其他成分组成 ， 转染 试 剂 与带负电荷的DNA相互作用，帮助其进入细胞内。 labome.cn | It is a proprietary mixture of positively charged lipids and other components that interact with the negatively charged DNA and allow for entry into the cell. labome.com |
慢病毒载体生产的第一步是将转运质粒、包膜质粒和gag-pol质粒与包装细胞 共 转染 （ 如果是逆转录病毒载体生产则为单 次 转染转 运 质 粒）。 labome.cn | The first step in lentiviral vector [...] production is co-transfection of the packaging cells with the transfer, envelope, and gag-pol plasmids (or single transfection of the transfer [...]plasmid in the [...]case of retroviral vector production). labome.com |
对逆转录病毒而言，转运载体被转染 至 Phoenix细胞内，Phoenix细胞是带有两个辅助构建体（env和gag-pol）作为附加体的人胚肾293T细胞。 labome.cn | For retroviruses, the transfer vector is transfected into Phoenix [...] cells, a cell line based on Human Embryonic Kidney 293T [...]that carries the two helper constructs (env and gag-pol) as episomes. labome.com |
包装细胞产生有感染能力的颗粒，其基因组仅编码来自于转运质粒的序列，这可用 于 转染 目 标 细胞。 labome.cn | The packaging cells produce infectious [...] particles, whose genome only encodes sequences [...] from the transfer plasmid, which can be used to transduce the target cells. labome.com |
原代细胞可以通过慢病毒或逆转录病毒载体（如引入 g f p 转染 的 癌细胞或肝细胞）进行转基因，然后导入小鼠体内。 labome.cn | Primary cells can be genetically modified using lentiviral or retroviral vectors (e.g. the introduction of gfp-transduced cancer cells or hepatocytes) and then introduced into mice. labome.com |
针对转染或基 因治疗，通过优化至纳米颗粒及脂质体上的加载，改善DNA或寡核苷酸的传输及半衰期。 malvern.com.cn | Improve the delivery and half-life of DNA or [...] oligonucleotides for transfection or gene therapy [...]by optimizing their loading onto nanoparticles and liposomes. malvern.com |
2001-2004 年期间，喀麦隆在接受治疗和强化新感染预防方面都实现了巨 大的进步，以期扭转传染病流 行的趋势。 daccess-ods.un.org | In the period 2001-2004, Cameroon achieved considerable progress, in terms of [...] improving access to treatment and intensifying [...] prevention of new infections, towards reversing the course of [...]the epidemic. daccess-ods.un.org |
复制缺陷的病毒是通过反式互补的方式生产的（图4），其中包装细胞与三种不同的质粒（见下文） 共 转染 （ 图 5），它们一起表达产生有感染能力的病毒颗粒所需的全部蛋白，并带有递送所需的目标核酸序列。 labome.cn | The production of replication defective virus is accomplished through trans-complementation (Figure 4) in which the packaging cells are cotransfected with three separate plasmids (Figure 5) (see below) that together [...] express all of the viral proteins necessary [...] to generate infectious particles, as [...]well as the nucleic acid sequence of interest [...]that will be packaged within them for delivery. labome.com |
为证实经遗传修饰的鸡X期胚盘细胞具有参与受体胚胎发育和形成嵌合体的能力，本研究将由鸡X期胚盘制成的细胞悬液与经脂质体包埋的抗鸡传染性支气管炎病毒基因重组质粒PGS1共孵育后，直接显微注入同期受体胚盘(140枚)；或 对 转染 后 供 体细胞进行G 418 抗性筛选后显微注入同期受体鸡胚盘(140枚)；或将供体细胞体外培养48 [...] h，再与脂质体-P GS1复合物共孵育后显微注入同期受体鸡胚盘(190枚)，制备转基因嵌合体鸡，并应用PCR和RAPD方法，对鸡胚和雏鸡不同组织或血液中的DNA进行检测。 actazool.org | Donor cells suspended from stage X [...] blastoderms and recombinant plasmid P GS1 [...] containing the infectious bronchitis [...]virus S1 gene were embedded in liposome and co cultured. actazool.org |
用RT-PCR和Western [...] blot测定PSP94和CRISP-3的表达，构建了PSP94和CRISP-3的哺乳动物表达，对重组蛋白的表达、定位和分泌进 行 转染 分 析 ，然后用Western blot和免疫荧光检测。 asiaandro.com | Reverse transcription-polymerase chain reaction and Western blot analysis were used to screen prostate cell lines for PSP94 and CRISP-3 expression. Mammalian expression constructs for human PSP94 and CRISP-3 were also generated and the expression, localization [...] and secretion of recombinant protein [...] were assayed by transfection followed by Western [...]blot analysis and immunofluorescence assay. asiaandro.com |
该工组组商定在乌兰巴托执行一项利用 空间技术监测空气污染和能量转换的 试点项目，并向讲习班组织方提交报告和 建议。 daccess-ods.un.org | The group agreed to implement a pilot project for [...] monitoring air pollution and energy transformation in Ulaanbaatar [...]using space technologies and [...]providing reports and recommendations to the workshop organizers. daccess-ods.un.org |
因此，环保部以及环保组织一直要求开展 污 染 物 排放 与 转 移 登 记制度(PRTR)的试点/示范工 作。 ecegp.com | Thus, environmental NGOs as well as MEP have been calling for the pilot / [...] demonstrations of pollution release and transfer registry (PRTR). ecegp.com |
同时制定并实施了一系列行业标准：《对受艾滋病毒感染人群保守治疗标 准》(2001 年)、《针对受艾滋病毒感染人群的抗 转 录 酶 病毒药物治疗和防止艾滋 病毒的母婴传播和职业感染的标准》(2001 年和 2003 年)、《艾滋病毒感染的流 行病学监测标准》(2002 年)、《艾滋病、肝炎和其他病毒感染实验室与诊断机构 组织实施抗击流行病计划操作指南》(2004 年)、《艾滋病毒母婴传播》单元 (2004 年)、《艾滋病毒检测咨询前后的方法指导》(2005 年)。 daccess-ods.un.org | Sector standards have been developed and implemented: “Palliative treatment of the persons infected with HIV and AIDS” (2001), “Treatment with antiretroviral medicine specific for persons infected with HIV and AIDS and the prophylaxis of transmission of the mother-child HIV infection, professional exposure” (2001 and 2003), “Epidemiologic supervision of the HIV/AIDS infection” (2002); Instructions on the organisation of the antiepidemiologic programme in the AIDS, hepatitis and other viral infections laboratories and diagnosis sections” (2004), The module “HIV and AIDS infections with mother-child transmission” (2004), Methodological Guide “Before and after HIV test counselling” (2005). daccess-ods.un.org |
马耳他完全致力于执行《巴塞罗那保护地中海海 [...] 洋环境和沿海区域公约》及其各项议定书，它们规定 缔约方有义务采取一切适当措施，以防止、应对并尽 [...] 可能消除地中海地区因船舶和飞机倾倒废物或是在 海上焚化废物、船舶排污、勘探和开发大陆架、海底 及其底土、陆上污染源以及越境转移 和 处置有害废物 受到污染。 daccess-ods.un.org | Malta is fully committed to the implementation of the Barcelona Convention for the Protection of the Marine Environment and the Coastal Region of the Mediterranean, as well as its Protocols, which obliges the contracting parties to take all appropriate measures to prevent, abate, combat and, to the fullest possible extent, eliminate pollution of the Mediterranean Sea area caused by dumping from ships and aircraft or incineration at sea, discharges from ships, exploration and exploitation of the continental [...] shelf and the seabed [...] and its subsoil, land-based sources and transboundary movements and the disposal [...]of hazardous wastes. daccess-ods.un.org |
在工业化市场经济国家中，比如美国、韩国以及日本，公开的 污 染 物 排放 与 转 移 清 单已将 投资人、生产商、消费者、政府、环保组织以及公共大众有效地团结在一起，环境表现欠佳的企 业在资本市场、产品市场都会受到惩罚，从而环境绩效好的企业能够实现环保与经济效益双赢 (Dasgupta 等人，2004；Konar 和 Cohen，1997；Decker 等人，2005；Agarwal 等人，2010)。 ecegp.com | In industrialized market economies such as the United States, Korea, and Japan, investors, producers, consumers, governments, environmental organizations, and the public have been effectively united by their publicly accessible pollutant release and transfer inventories to deliver both environmentally and economically desirable outcomes (Dasgupta et al. 2004; Konar and Cohen 1997, 2001; Decker et al. 2005; Agarwal et al. 2010). ecegp.com |
(a) 制訂新法例，進㆒步減低空氣、噪音和水 污 染 ， 包 括：管制手提撞擊式破碎機； 增加空氣污染罪行的刑罰和簡化有關發出消減空氣 污 染 滋 擾 通知書的手續；管 制石棉的使用；管制廢物處理裝置的使用和維修，並訂立條文規定樓宇業主將 廢物排放入公共污水渠；設立㆒項收費制度，就處理工業污水，使之符合標準 而徵收費用，以及立例管制化學廢物的處理，並徵收費用。 legco.gov.hk | They cover controls of hand held percussion breakers; increases in penalties and improvements in the procedure for abatement notices to air pollution offences; [...] controls on the use of asbestos; [...]controls on the use and maintenance of waste treatment plants, provision to direct property owners to discharge wastes into public sewers; for a system of charging for treating of industrial effluent to specified quality and regulations to control the disposal of chemical waste and charge for it. legco.gov.hk |
(17) 經營所有或任何涉及以任何方式預備、紡織、編織、梳理、沖刷、裁切、漂 白、著色、染色、 印刷及整理或製造棉、羊毛、絲、亞麻、麻、亞麻布、黃 麻、皮革、合成纖維及其他纖維或紡織品（不論屬任何狀態下之動物、植物 或礦物纖維及不論是否類似上述物質），及處理及使用及買賣任何來自任何該 等業務（不論由本公司或其他人士進行）之廢料之行業或業務，亦生產硫酸 鹽及漂白及染整物 料以及買入及賣出及買賣上述所有或任何物質。 equitynet.com.hk | (17) To carry on all or any of the trades or business of preparing, spinning, knitting, doubling, weaving, combing, [...] scouring, sizing, [...] bleaching, colouring, dyeing, printing and finishing, working or manufacturing in any way whatever, cotton, wool, silk, flax, hemp, linen, jute, leather, synthetic fibres and other fibrous or textile substances, whether animal, vegetable or mineral in any state and whether similar to the foregoing substances or not and to treat and utilise and deal in any waste arising from any such operations, whether carried out by the Company or otherwise, and also of makers of vitriol and of bleaching, dyeing, and finishing materials, [...]and the buying [...]and selling of and dealing in all or any of the aforesaid substances. equitynet.com.hk |
联合国驻利比里亚特派团部队将合并地点和基地 ， 转 为 更 机动的态势，并在 特派团军事部门减员的情况下开展工作，以便为该国各地提供安全防护，支持国 家安全部队，与新组建的军队举行联合演习，按照联合国军火禁运豁免规定检查 政府获得的武器和弹药库存，供国家警察使用，开展警戒和搜查行动以收回非法 武器，保护联合国和政府所在地点和设施，并定期进行空中和地面巡逻，特别是 对边界沿线和其他高风险地区进行巡逻。 daccess-ods.un.org | The forces of the United Nations Mission in Liberia will consolidate their locations and bases, shifting to a more mobile posture and working within the reduced strength of the military component of the Mission, in order to provide a security umbrella throughout the country, support the national security forces, conduct joint exercises with the new army, undertake inspections of inventories of weapons and ammunition obtained by the Government in accordance with the exemptions to the United Nations arms embargo for use by the national police, undertake cordon and search operations to retrieve illegal weapons, protect key United Nations and Government locations and installations and conduct regular air and ground patrols, in particular along the borders and in other high-risk areas. daccess-ods.un.org |
除在议程项目 3 下审议的共同文件外，经社会还收到贸易和投资委员 [...] 会第二届会议的报告(文件 E/ESCAP/68/6)、亚洲及太平洋技术转让中 心理事 会第七届会议的报告(文件 E/ESCAP/68/7)、以及联合国亚洲及太平洋农业工 [...] 程和机械中心理事会第七届会议的报告(文件 E/ESCAP/68/8)。 daccess-ods.un.org | In addition to the common documents being considered under agenda item 3, the Commission had before it the report of the Committee on Trade and Investment on its second session (E/ESCAP/68/6), the report of [...] the Governing Council of the Asian and [...] Pacific Centre for Transfer of Technology on its [...]seventh session (E/ESCAP/68/7) and [...]the report of the Governing Council of the United Nations Asian and Pacific Centre for Agricultural Engineering and Machinery on its seventh session (E/ESCAP/68/8). daccess-ods.un.org |
因此，会议促请拥有先进生 物技术的所有缔约国采取积极措施，促进在平等和无歧视基础上开展技 术 转 让和 国际合作，尤其是与该领域中不那么先进的国家开展技 术 转 让 和 国际合作，同时 促进《公约》的基本目标，并确保科学技术的普及完全符合《公约》的和平目标 [...] 和宗旨。 daccess-ods.un.org | Therefore, the Conference urges all States Parties [...] possessing advanced [...] biotechnology to adopt positive measures to promote technology transfer and international cooperation on an equal and non-discriminatory [...]basis, particularly [...]with countries less advanced in this field, while promoting the basic objectives of the Convention, as well as ensuring that the promulgation of science and technology is fully consistent with the peaceful object and purpose of the Convention. daccess-ods.un.org |
在这方面，咨询委员会收 到一张表(见表八.8.)，显示联合国所有租赁房地产的租约到期日、年租金估计 数和面积，并在设施管理处签订的长期租约、基本建设总计划 周 转 空 间 、设施管 理处租用的基本建设总计划周转空间 和设施管理处为“团结”项目租赁的房地产 之间进行了区分。 daccess-ods.un.org | In this connection, the Advisory Committee was provided with a table (see table VIII.8) showing the lease expiry, estimated annual rental costs and square footage of all United Nations leased properties, distinguishing between longterm leases concluded by the Facilities Management Service, capital master plan swing spaces, Facilities Management Service leases in the capital master plan swing spaces and properties leased by Facilities Management Service for the Umoja project. daccess-ods.un.org |
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