| 单词 | 质粒 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
| 释义 | 质粒 noun —plasmids plSee also:粒 n—particles pl • grain n 粒—granule • classifier for small objects, such as a grain of rice
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例如，使用相同或不同的 构建质粒对同 一植物物种的两个不同品系进行转基因会产生两个转基因品系。 excellencethrou...stewardship.org | For example, two lines of the same plant species transformed with the same or different constructs constitute two events. excellencethrou...stewardship.org |
遗传物质包括染色体、基因组、质粒 、 转位子和载体(包括转基因的、非转 [...] 基因的、以及整体或部分化学合成的)。 daccess-ods.un.org | Genetic elements include inter alia [...] chromosomes, genomes, plasmids, transposons, [...]and vectors whether genetically modified [...]or unmodified, or chemically synthesized in whole or in part. daccess-ods.un.org |
该系统以大肠杆菌 K12菌株和一系列高表达质粒为基础。 wacker.com | It is based on an E. coli K12 strain and a [...] series of highly expressive plasmids. wacker.com |
确定特性粘度，以便推断DNA质粒是否 为线性、螺旋性或超螺旋性（单/双-链，片段）。 malvern.com.cn | Determine the intrinsic viscosity to [...] infer if the DNA plasmid is linear, coiled [...]or super coiled (single/double-stranded, fragments). malvern.com |
在本模块中，贴记标签是指附加标记了用于确认构 建 质粒 或 转化株材料特征的名称和／或其它鉴别信息(例如： 条形码）的标签。 excellencethrou...stewardship.org | For the purposes of this module, labelling means to affix with a label that is marked with a name and/or other identifying information (e.g. bar code) that can be used to confirm construct or transformant identity. excellencethrou...stewardship.org |
其后不久，他们又证明了只需把动物细胞的D NA 与 质粒 连 结，细胞的DNA便可以在细菌内繁殖，而制造载体核糖核酸(RNA)的细胞系统亦可以转录这外来的DNA。 shawprize.org | Soon afterward, Boyer and Cohen collaborated to show that DNA from [...] animal cells can be propagated in bacteria by [...] linking it to plasmids, and that the [...]cellular machinery that makes messenger RNA [...]can transcribe this foreign DNA. shawprize.org |
通过使质粒与特 殊的生产菌株相结合，我们能够从开发阶段的源头对后续的生产和提纯工艺进行优化和简化， [...] 从而实现了药用蛋白的高效和低成本制造。 wacker.com | By combining plasmids and the special [...] production strain, the subsequent production and purification processes can be streamlined [...]and simplified from the earliest phases of development. wacker.com |
注意：当结合Fugene和质粒时，建议使用聚苯乙烯管，Fugene最后添加以避免与管子相互作用。 labome.cn | Note: When [...] combining Fugene and plasmids, it is suggested [...]that polystyrene tubes be used and the Fugene be added last to [...]avoid it interacting with the tube. labome.com |
在制备感受态细胞和大量提取质粒DNA 时 ，经常使用到自动高速冰冻离心机和超高速冷冻离心机型;如需要大量离心，例50mL、100mL或100mL以上，且转速需要20000～70000rpm，则可以使用自动超速冰冻离心机。 xybjb.com.cn | In the preparation of extraction and feel [...] normal cellular plasmid DNA, often use [...]to automatic high-speed freezing centrifuge [...]and ultra-high speed frozen centrifugal model; If need a large number of centrifugal, an example 50 mL, 100 mL or 100 mL above, and speed to 20000 ~ 70000 RPM, one may use the automatic speed centrifuge frozen. xybjb.com.cn |
科恩和布瓦耶在一次科学会议中会面，并且合作设计了一套方法，把连接不 同 质粒 的 DNA碎片，以及活细胞内DNA复合分子的繁殖和生物功能等问题一起解决过来。 shawprize.org | Cohen and Boyer met at a scientific meeting and designed a method for [...] accomplishing both the joining of DNA fragments [...] from different plasmids, and the propagation [...]and biological functioning of the [...]composite DNA molecules in living cells. shawprize.org |
α-银环蛇毒素在神经科学研究以及在临床和制药上有重要作用，为获得大量的α-银环蛇毒素，我们用已构建的pGEX-BgTX（P22-A 31 ） 质粒 在 大 肠杆菌BL21（DE3）中表达，并用谷胱甘肽Sepharose [...] FF纯化GST-α-银环蛇毒素融合蛋白，再用凝血酶切掉融合标签谷胱苷肽转移酶（Glutathione [...]S-transferase，GST），得到了较纯的重组α-银环蛇毒素同工毒素，得率约为1.225 mg/L。 actazool.org | In order to acquire quantitites of α-bungarotoxin, we [...] expressed GST-α-bungarotoxin fusion [...] protein using constructed plasmid pGEX-BgTX (P22-A31) [...]in E.coli BL21 (DE3)cell, purified GST-α-bungarotoxin [...]using glutathione-Sepharose FF, cleavaged Glutathione S-transferase(GST) tag by thrombin in the column, and finally, we obtained recombinant α-bungarotoxin with yields of about 1.225 mg/L. In order to compare the immunogenicity of recombinant protein and crude α-bungarotoxin, we prepared multi-antibody using recombinant α-bungarotoxin. actazool.org |
按照人金属硫蛋白-3(hMT-3)的基因序列,选用大肠杆菌偏爱的密码子合成了全长hMT-3基因,并将其插入大肠杆菌融合表 达 质粒 p A L EX的多克隆位点中,在谷胱甘肽-硫-转移酶(GST)下游与GST融合表达。 chrom-china.com | The gene encoding human metallothionein-3 (hMT-3) was synthesized and inserted into the poly-cloning sites of fusion expression vector pALEX, and fused downstream to its glutathione S-transferase (GST) fusion partner. chrom-china.com |
复制缺陷的病毒是通过反式互补的方式生产的（图4），其中包装细胞与三种不同 的 质粒 （ 见 下文）共转染（图5），它们一起表达产生有感染能力的病毒颗粒所需的全部蛋白，并带有递送所需的目标核酸序列。 labome.cn | The production of replication defective virus is accomplished through trans-complementation (Figure 4) in which [...] the packaging cells are cotransfected with [...] three separate plasmids (Figure 5) (see [...]below) that together express all of the [...]viral proteins necessary to generate infectious particles, as well as the nucleic acid sequence of interest that will be packaged within them for delivery. labome.com |
为证实经遗传修饰的鸡X期胚盘细胞具有参与受体胚胎发育和形成嵌合体的能力，本研究将由鸡X期胚盘制成的细胞悬液与经脂质体包埋的抗鸡传染性支气管炎病毒基因重 组 质粒 P G S 1共 孵育后，直接显微注入同期受体胚盘(140枚)；或对转染后供体细胞进行G 418 抗性筛选后显微注入同期受体鸡胚盘(140枚)；或将供体细胞体外培养48 h，再与脂质体-P GS1复合物共孵育后显微注入同期受体鸡胚盘(190枚)，制备转基因嵌合体鸡，并应用PCR和RAPD方法，对鸡胚和雏鸡不同组织或血液中的DNA进行检测。 actazool.org | Donor cells suspended from stage X blastoderms and recombinant plasmid P GS1 containing the infectious bronchitis virus S1 gene were embedded in liposome and co cultured. actazool.org |
2 h,细胞生长较快;不同代数的染色体众数为2n = [...] 74;免疫组织化学鉴定波形蛋白抗体呈阳性,角蛋白抗体呈阴性;外 源 质粒 能 在 该细胞内整合和正确表达;微生物污染检测呈阴性。 mammal.cn | The results showed that the population doubling time (PDT) of the cells was 47 2 h;the majority chromosome number was 74;the immunocytochemistry was [...] positive for vimentin and negative for [...] cytokeratin;an exogenous plasmid could integrate [...]and express in the cells;detection for microbial contamination was negative. english.mammal.cn |
阿基米德是一款创新型、高性能系统，利用共振质量测量技术，对粒度范围在50nm~5μm的颗粒进行检测与计数，同时测量 颗 粒质 量 及 粒 度。 malvern.com.cn | Archimedes is an innovative, high-performance system which harnesses the technique of resonant [...] mass measurement in order [...] to detect and count particles in the size range 50nm - 5µm and to measure particle mass and size. malvern.com |
高级格式化标准中的较大 4K 扇区可加倍2 EEC [...] 块大小，从 50 字节到 100 字节，带来针对颗粒和介质缺陷 的错误纠正效率和稳健性方面必要的改进。 seagate.com | The larger 4K sector in the Advanced Format standard approximately doubles2 the size of the ECC block from 50 bytes to 100 [...] bytes, providing a much needed improvement in error correction efficiency and [...] robustness against particles and media defects. seagate.com |
分离器在给料的固体颗粒质量密 度为25%时工作效率最佳。 zs.weirminerals.com | Separators operate best at a feed density of <25% solids by mass. de.weirminerals.com |
相关的观测包括但不 限于温室气体、悬浮颗粒和空气质量 参数的测量及其变化情况；植被和土地利 用，毁林情况，包括森林火灾、土地退化和荒漠化造成的毁林情况；森林生物 量的变化；生物多样性的丧失；冰川后退和波动；以及海平面高度测量、降 水、云和全球水循环的变化。 daccess-ods.un.org | Related observations included, but were not limited to, measurements of and changes in greenhouse gases, aerosols and air quality parameters; land cover and land use, deforestation, including from forest fires, land degradation and desertification; changes in forest biomass and loss of biodiversity; glacial retreat and surges; and sea surface altimetry, precipitation, clouds and global water circulation changes. daccess-ods.un.org |
会议建议，缔约国应思考如何使各方能够就请求 开展必要的深入分析和讨论，以确保提出 高 质 量 的 请求仍然是规范做法。 daccess-ods.un.org | It was suggested that the States Parties reflect on how they can enable the necessary [...] in-depth analysis and discussion of the requests in order to [...] ensure that high quality requests continue [...]to be the norm. daccess-ods.un.org |
在 “本港”之前加上“鑒於”；在“日趨惡化，”之後加上“尤其在二氧 化硫及微細懸浮粒子的 排放方面，”；在 “資助等；”之後刪除“及 [...] ”； 及在緊接句號之前加上“； (八 ) 在啟德發展區以外，廣泛推廣應用 區域供冷系統，以便提高能源效益，達致減排效果；及(九 [...] ) 改善 城市規劃及設計，以改善市區的空氣流通，減少空氣污染物於市 區積聚，例如加快檢討各區的《分區計劃大綱圖》以修訂規劃參 數、制 訂 ‘空氣流通目標’、研究將‘空氣流通評估’和 ‘空氣流通指引’ 訂為法定要求，以加強規範私人發展項目對周邊環境的影響”。 legco.gov.hk | To add ", as" after "That"; to add "particularly [...] regarding the emission of sulphur [...] dioxide and fine suspended particulates," after "the [...]day,"; to delete "and" after "under [...]the schemes;"; and to add "; (h) in areas other than the Kai Tak Development Area, widely promote the use of district cooling system to enhance energy efficiency and achieve emission reduction; and (i) improve town planning and urban design to improve air ventilation and reduce the accumulation of air pollutants in urban areas, such as expediting the review of the Outline Zoning Plans of various districts to revise the planning parameters, formulating 'air ventilation objectives', studying making 'air ventilation assessment' and 'air ventilation guidelines' statutory requirements, so as to strengthen the regulation of the impact of private developments on the surrounding environment" immediately before the full stop. legco.gov.hk |
政府當局已採取各項措施改善路邊空氣質素，包括收緊車 用燃料及新登記車輛的廢氣排放標準、引入石油氣車輛取代柴油 [...] 的士和小巴、規定所有歐盟前期柴油車輛加裝 微 粒 消 減裝置、提 高黑煙車輛的排放標準和測試方法、推出資助計劃鼓勵使用環保 [...] 車輛和更換歐盟III期前的柴油商業車輛。 legco.gov.hk | These include tightening the standards for motor vehicle fuels and the emissions of newly registered vehicles, introducing LPG vehicles to replace diesel taxis and light buses, [...] mandating all pre-Euro diesel vehicles to be [...] retrofitted with particulate reduction devices, [...]upgrading the standard and test procedures [...]for vehicle smoke emissions, launching incentive schemes to encourage the use of environment-friendly vehicles and replacement of pre-Euro III diesel commercial vehicles. legco.gov.hk |
挂图中的数据包括农村地区的农村人口规模和增长速度、农业人口规模、 农业区和农田、森林面积、灌溉面积、获得改善供水和卫生的情况、农业中经济 [...] 活跃人口和化肥的使用；以及城市地区的城市人口规模和增长率、人类住区占据 [...] 的土地面积、生活在贫民窟中的城市人口、获得改善供水和卫生的情况、二氧化 碳的排放量、空气中颗粒物的 密度、产业和服务业中的就业、国内生产总值、使 [...]用中的机动车辆和能源消耗。 daccess-ods.un.org | The data contained in the wallcharts include, for the rural areas, size and rate of growth of the rural population, size of the agricultural population, agricultural area and cropland, forest area, irrigated areas, access to improved water and sanitation, economically active population in agriculture and fertilizer use; and for the urban areas, size and rate of growth of the urban population, land area occupied by human settlements, urban population living in slums, access to improved water and [...] sanitation, carbon dioxide emissions, [...] concentrations of particulate matter in the air, [...]employment in industry and services, [...]gross domestic product, motor vehicles in use and consumption of energy. daccess-ods.un.org |
大会第六十六届会议强调指出继续对《关于进一步执行小岛屿发展中国家可 持续发展行动纲领的毛里求斯战略》的后续行动和执行情况进行 实 质 性 审 议的重 要性，并决定在其第六十七届会议上审议为第六十六届会议印发的秘书长关于更 好地执行《小岛屿发展中国家可持续发展的行动纲领》和《关于进一步执行小岛 屿发展中国家可持续发展行动纲领的毛里求斯战略》的具体建议的报告 (A/66/278)以及秘书长关于审查联合国系统对小岛屿发展中国家支持情况的报 告(A/66/218)(第 66/198 号决议)。 daccess-ods.un.org | At its sixty-sixth session, the General Assembly stressed the importance of the continued substantive consideration of the follow-up to and implementation of the Mauritius Strategy for the Further Implementation of the Programme of Action for the Sustainable Development of Small Island Developing States and decided to consider at its sixty-seventh session the reports of the Secretary-General on concrete recommendations to enhance the implementation of the Barbados Programme of Action for the Sustainable Development of Small Island Developing States and the Mauritius Strategy for the Further Implementation of the Programme of Action for the Sustainable Development of Small Island Developing States (A/66/278) and on the review of United Nations system support to small island developing States (A/66/218), issued for the sixty-sixth session (resolution 66/198). daccess-ods.un.org |
优质教育 的下列方面受到了重视：支持课程体系的审查 和修订，特别是根据各国的优先工作和阿拉伯世界的需求纳入价值观教育和伦理教育；“以 学习者为中心”的教育；改进教学工作，包括能力培养和教师培训；提高教师地位；制定标 准进行评估；制订关于优质教育 的国家标准。 unesdoc.unesco.org | The following elements of quality education were highlighted: focus on assistance in curricula review and revision, particularly the introduction of values education and ethical approaches, in accordance with national priorities and Arab needs; “learner-centred” education; improvement of teaching including capacity-building and teacher training; improvement in the status of teachers; development of benchmarking and assessment; elaboration of national standards regarding quality education. unesdoc.unesco.org |
加拿大的包括圣卢西亚制冷和空调行业的消耗臭氧层 物 质 的 最 终淘汰管理计划和乌拉 圭附件 A 第一类物质最终 淘汰管理计划，意大利的是刚果民主共和国最终淘汰溶剂行业的 消耗臭氧层物质总体项目，摩洛哥淘汰用作土壤熏蒸剂的甲基溴，在塞尔维亚最终淘汰四 氯化碳项目；西班牙的是阿拉伯利比亚民众国淘汰园艺中的甲基溴。 multilateralfund.org | For Canada, those covered the terminal phase-out management plan for ODS in the refrigeration and air-conditioning sector [...] in Saint Lucia and the [...] terminal phase-out management plan for Annex A Group I substances in Uruguay; for Italy, the umbrella project for terminal phase-out of ODS in the solvent sector in the Democratic Republic of Congo, the phase-out [...]of methyl bromide [...]as a soil fumigant in Morocco, and the terminal CTC phase-out project in Serbia; and for Spain, the phase-out of methyl bromide in horticulture in Libyan Arab Jamahiriya. multilateralfund.org |
值得注意的是，本办事处在教育领域开展的计划主要关注以下战略支助领域，所有这 [...] 些都直接回应肯尼亚教育部门支助计划（KESSP）所载的战略和成果，教育部门计划实现 《2030 年展望》：通过教育管理信息系统加强规划、监测和评估教育系统；加强教育部门 [...] 以应对艾滋病毒和艾滋病；提高教育促和平计划 的 质 量 ；以及通过宣传和技术支持战略规划 [...]来加强扫盲发展。 unesdoc.unesco.org | It is noteworthy that programmes implemented by the Office in the area education focused on the following strategic areas of support, all directly responding to strategies and results contained in Kenya Education Sector Support Programme (KESSP), the education sector’s plan to materialize Vision 2030: strengthening the planning, monitoring and evaluation of the education system through EMIS; enhancing the [...] education sector responses to HIV and AIDS; [...] improving the quality of education [...]for peace programme; and strengthening literacy [...]development through advocacy and technical support to strategic planning. unesdoc.unesco.org |
英汉双解词典包含2273206条英汉词条，基本涵盖了全部常用单词的翻译及用法，是英语学习的有利工具。