| 单词 | 磷酸化 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| 释义 | 磷酸化 noun —phosphorylation nSee also:磷酸 n—sulfate n • sulphate n • trisodium phosphate n • sodium pyrophosphate n 磷 adj—phosphorus adj 磷化 n—phosphide n 酸化 n—acidification n
|
磷酸化，或 丝氨酸，苏氨酸或酪氨酸残基增加一个磷酸基团，是蛋白质修饰最常见的形式之一(图3A)。 labome.cn | Phosphorylation, or the addition of a phosphate group to serine, [...] threonine, or tyrosine residues, is one of most common [...]forms of protein modification (Fig 3A). labome.com |
然后，反应液用滤膜过滤，蛋白质结合到滤膜，洗去未结合的ATP，用闪烁计数器检 测 磷酸化 水 平（保留在滤膜的放射性同位素）。 labome.cn | The reaction solution is then passed through a filter to bind [...] protein but wash away unbound ATP, and [...] the level of phosphorylation (radioisotope [...]retained on filter) is detected by a scintillation counter. labome.com |
運動員體內的磷酸鹽是被用來在肝醣合成過 程中提昇葡萄糖利用率的一個工具，這個合成過程是需 要 磷酸化 的 輔 助。 herbalifenutritioninstitute.com | Phosphate salts in the athlete are also meant to enhance glucose utilization for glycogen [...] synthesis, which requires phosphorylation. herbalifenutritioninstitute.com |
这个磷酸基团可以被用作标记，无论是使用放射性磷酸盐进行放射性测量，或是使用针 对 磷酸化 氨 基 酸的抗体。 labome.cn | This phosphate group can be used as a marker, either in [...] using radioactive phosphate for radiometric assays, or by utilizing antibodies [...] raised against the phosphorylated amino acid. labome.com |
放射测量激酶反应使用32P-gamma-ATP，可用于检测体 外 磷酸化 状态 [8] 。 labome.cn | Radiometric kinase reactions using 32P-gamma-ATP can be [...] used to assay phosphorylation status in vitro [8] . labome.com |
用计算机方法或用质谱法识别了（假定） 的 磷酸化 位 点 之后，可以从公司购买针 对 磷酸化 位 点的通用抗体，通过免疫沉淀的步骤，以确定目标蛋白质是否在特定类型的位点 被 磷酸化 （ 例 如一个典型的cyclin/cdk位点） [10] 。 labome.cn | Following identification of a [...] (putative) phosphorylation site by an in silico method or by mass spectrometry, commercially available antibodies for the general class of phosphorylation sites can be used in conjunction with an immunopercipitation step to determine if the protein of interest is phosphorylated at a specific [...]type of site (for example [...]a canonical cyclin/cdk site) [10] . labome.com |
由于磷酸基等分子的分子量和电荷是已知的，所以也可以检测肽段中特定氨基酸 的 磷酸化。 labome.cn | The molecular weight and charge of a moiety such [...] as a phosphate group is also [...] known, the phosphorylation of specific amino acids within a peptide [...]fragment can also be assessed. labome.com |
在这里，我们重点介绍识别蛋白质修饰的一般方法，质谱；识 别 磷酸化 的 特 异性方法，磷酸盐标记；识别泛素化的方法；在染色质重塑过程中识别组蛋白乙酰化和甲基化的方法；识别蛋白质糖基化的方法（表1和图1B）。 labome.cn | Here we focus on the mass spectrometry as a general approach for identifying protein modification, and specifici methods for phosphorylation, such as orthophosphate labeling, for ubiquitination, for histone acetylation and methylation in the context of chromatin remodeling, and for protein glycosylation (Table 1 and Fig 1B). labome.com |
亨特教授于1979年发现多瘤病毒中T抗原和劳斯肉瘤病毒的V-Src致癌基因都显示出一种前所未知的蛋白激酶活性，引致酪 氨 酸磷酸化。 shawprize.org | In 1979, he discovered that polyomavirus middle T antigen and the RSV v-Src oncoprotein both exhibit a previously unknown protein kinase activity that phosphorylates tyrosine. shawprize.org |
蛋白磷酸酶抑制剂冈田酸可以克服CHX+A23187对小鼠卵母细胞活化作用，并且部分阻止MA PK 去 磷酸化。 actazool.org | Egg activation induced by CHX+A23187 was [...] suppressed by okadaic acid, and MAP kinase [...]dephosphorylation was partially reversed as well. actazool.org |
几种体内的和体外的方法，被用于检测蛋白质 的 磷酸化 状 态 ，检测一个特定氨基酸 的 磷酸化 ， 以 及确定一个特定的激酶（或磷酸酶）是否作用于目标蛋白质。 labome.cn | Several in vivo and in vitro methods are used to assess protein phosphorylation status, to assess phosphorylation of a specific amino acid, and to ascertain whether a specific kinase (or phosphatase) modifies a protein in question. labome.com |
也可以使用针对一个特定的磷酸化氨 基 酸， 如 磷酸化 酪 氨 酸，制备的抗体(图3B)。 labome.cn | Antibodies generated against a specific phosphorylated amino acid, such as a phosphotyrosine, can also be used (Fig 3B). labome.com |
保守的核心组蛋白H2A，H2B，H3，和H4的表观遗传修饰（ 如 磷酸化 ， 泛 素化，乙酰化和甲基化）在基因表达中发挥重要的调节作用 [...] [15] 。 labome.cn | Epigenetic [...] modifications (such as phosphorylation, ubiquitination, [...]acetylation and methylation) of the conserved core histones [...]H2A, H2B, H3, and H4 play an important regulatory role in gene expression [15] . labome.com |
实验结果显示,含Ti和Si物质的量比为0.08的Ti-SBA-15介孔材料可选择性地对β-酪蛋白酶解产物中 的 磷酸化 肽 进行选择性富集;对于β-酪蛋白和牛血清白蛋白物质的量比为1:100的蛋白质酶解混合液,Ti-SBA-15仍能实现对 其 磷酸化 肽 的 有效富集。 chrom-china.com | The matrix assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) analysis showed that [...] the [...] phosphopeptides of β-casein digest could be selectively enriched by the Ti-SBA-15 with Ti/Si molar ratio of 0.08, even under the interference of bovine serum albumin (BSA) with the molar ratio of β-casein [...]to BSA up to 1:100. chrom-china.com |
这种方法是半定量，但可以确定 磷酸化 蛋 白 的分子量。 labome.cn | This method is less quantitative, but can confirm the molecular [...] weight of the phosphorylated protein. labome.com |
第一类是通用的 磷酸化酪氨酸，磷酸化丝氨酸， 磷酸化 苏 氨 酸抗体，将结合于任 何 磷酸化 酪 氨 酸 ， 磷酸化 丝 氨酸 和 磷酸化 苏 氨 酸分子，与邻近的氨基酸残基无关。 labome.cn | One category is the generic phosphotyrosine, phosphoserine, and phosphothreonine antibody, which will bind to any phospho-tyrosine, phospho-serine, and phospho-threonine [...] moeities regardless the [...]neighboring amino acid residues. labome.com |
上述结果表明,作为一种多孔、高比表面积 的 磷酸化 多 肽的选择性吸附材料,Ti-SBA-15有望 在 磷酸化 蛋 白质组的分析中得到广泛的应用。 chrom-china.com | It can be concluded that the Ti-SBA-15 showed the specific adsorption toward the phosphorylated peptides, which provides great potential in the specific capture of phosphopeptides. chrom-china.com |
这种技术被用于确定带有这种修饰的‘次级蛋白质组’，例 如 磷酸化 蛋 白 质组分析癌症中整个信号网络的激活状态 [6] 。 labome.cn | Such techniques are used to define a ‘sub-proteome’ bearing this modification, such as phosphoproteom for analyzing the activation state of whole signaling networks like in cancer [6] . labome.com |
对于激酶和磷酸化蛋白 来说，相关技术来自斯坦福大学的Gary Nolan 实验室 [6] [7] 。 labome.cn | For kinases and phosphoproteins, the technology comes from the laboratory of Gary Nolan at Stanford [6] [7] . labome.com |
除了被用来确定单一蛋白质的修饰状态，质谱还被用来确定带有某种特定修饰 如 磷酸化 的 所 有蛋白质的广泛数据集。 labome.cn | In addition to be used to determine the modification state of a single protein, mass [...] spectrometry has been used to generate extensive datasets of proteins that carry a specific [...] modification, such as phosphorylation. labome.com |
用一组新颖的特征来表示所被预测的氨基酸位点周围的肽链，并且使用基于特征选择的支持向量机来预测某一丝氨酸或苏氨酸位点是否会潜在地 被 磷酸化。 chinese.eurekalert.org | In this work, the authors propose to use a unique set of features to represent the peptides surrounding the amino acid sites of interest and use feature selection support vector machine to predict whether the [...] serine/threonine sites are potentially [...] phosphorylable, as well as selecting important features that may lead to phosphorylation. chinese.eurekalert.org |
识别细胞周期蛋白（聚合酶的裂解结构 和 磷酸化 组 蛋白H3）的抗体都可以购买到。 labome.cn | Antibodies to the cyclins, the cleaved form of PolyPolymerase [...] (PARP) and phosphorylated histone H3 [...]are commercially available. labome.com |
根据目标抗体是否分泌并在高尔基体内滞留，激酶 或 磷酸化 蛋 白是否需要磷酸酶抑制剂，核蛋白是否需要磷脂双分子层部分溶解和DNA松弛，细胞透化需要选择不同的方式。 labome.cn | There are different choices for cell permeabilization depending on whether the [...] antibody target is to be secreted [...] and therefore sequestered in the Golgi, a kinase or phosphoprotein [...]that requires aggressive [...]inhibition of phosphatases, or a nuclear protein that requires partial dissolution of two membranes and relaxation of the DNA. labome.com |
第二类包括的抗体是磷酸化特定 氨基酸表位抗体 (参见 摘要 在来邦网产品数据库的磷酸化特异 性抗体)。 labome.cn | The second catogery includes the antibodies again phospho-specific amino acid epitopes (see a summary of phospho-specific antibodies [...] in Labome product database). labome.com |
A,磷酸化过程 中，一个磷酸基团被结合到丝氨酸，苏氨酸或酪氨酸残基。 labome.cn | A, During phosphorylation, a phosphate group is [...] added to a serine, threonine, or tyrosine residue. labome.com |
香港大學研究小組正作進一步研究，以評估單獨使用S TA T3 磷酸化抑致劑（STAT3 phosphorylation [...] inhibitors）或將之結合傳統化療，哪一種對於抑制肝癌復發的治療效果較佳，目的是開發分子標靶藥物，以有效地消除這種致命的疾病。 xn--pssu7cv61a...xn--wcvs22d.hk | The HKU research team is conducting a further [...] study to evaluate the therapeutic [...] efficacy of STAT3 phosphorylation inhibitors alone [...]in the suppression of liver cancer [...]recurrence and its combined effect with conventional chemotherapy, with the aim of developing molecular targeted drugs to effectively eradicate this deadly disease. xn--pssu7cv61a...xn--wcvs22d.hk |
B, 翻译后修饰，作为细胞内信号（ 磷酸化 ） ， 蛋白稳定性的调控（泛素），转录调控（组蛋白乙酰化和甲基化），细胞表面信号（糖基化）等多种多样的细胞功能中发挥重要作用。 labome.cn | B, Post-translational modifications play important roles [...] in diverse cell functions as intracellular [...] signaling (phosphorylation), regulation [...]of protein stability (ubiquitination), [...]regulation of transcription (histone acetylation and methylation), and cell surface signaling (glycosylation). labome.com |
研究亦發現，CD24+標誌的癌幹細胞是透過激活STAT3 [...] 訊號去維持肝癌幹細胞自我更新和使腫瘤形成，因此，研究人員相信透過抑制S TA T3 磷酸化可大大提升肝癌治療效果。 xn--pssu7cv61a...xn--wcvs22d.hk | The study also finds that CD24+ maintains self-renewal of CSCs and forms tumours through activating STAT3 [...] signals, as such, the researchers believe that through [...] inhibiting STAT3 could improve treatment [...]efficacy for liver cancer. xn--pssu7cv61a...xn--wcvs22d.hk |
结论PCOS [...] IR可能为胰岛素受体后信号转导通路中，其上游信号分子IRS-1酪 氨 酸磷酸化 的 改变造成激活信号减弱，使PI-3K活性降低，影响胰岛素信号通路中信号分子的生物学作用，导致IR形成。 zhhyx.com.cn | Conclusion In consequence of the weaker signal caused by the [...] change of upper stream signal molecule [...] IRS-1 tyrosine phosphorylation, PI-3 kinase [...]activity decreased, it affects the insulin [...]signal pathway leading to insulin resistance in PCOS. zhhyx.com.cn |
氯磷酸二乙酯; 氯代磷酸二乙酯; 氯化磷酸二乙酯 chemblink.com | Diethyl phosphorochloridate; Phosphorochloridic acid diethyl ester chemblink.com |
英汉双解词典包含2273206条英汉词条，基本涵盖了全部常用单词的翻译及用法，是英语学习的有利工具。