| 单词 | 慢病毒 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| 释义 | 慢病毒 adjective —lentiviral adjSee also:慢 adj—slow adj 慢 adv—slowly adv 慢慢 adv—gradually adv 病毒 n—virus n • infection n • viruse n
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关于处理慢病毒和逆转录病毒的更多信息和实验室生物安全水平标准，请询问NIH和美国疾病控制与预防中心健康和安全办公室。 labome.cn | Further information [...] about handling lentiviruses and retroviruses, [...]and a description of laboratory biosafety level criteria, [...]is provided by the NIH and the Centers for Disease Control Office of Health and Safety:. labome.com |
慢病毒载体生产的第一步是将转运质粒、包膜质粒和gag-pol质粒与包装细胞共转染（如果是逆转录病毒载体生产则为单次转染转运质粒）。 labome.cn | The first step in lentiviral vector production [...] is co-transfection of the packaging cells with the transfer, envelope, and [...]gag-pol plasmids (or single transfection of the transfer plasmid in the case of retroviral vector production). labome.com |
原代细胞可以通过慢病毒或逆转录病毒载体（如引入gfp转染的癌细胞或肝细胞）进行转基因，然后导入小鼠体内。 labome.cn | Primary cells can be genetically [...] modified using lentiviral or retroviral [...]vectors (e.g. the introduction of gfp-transduced [...]cancer cells or hepatocytes) and then introduced into mice. labome.com |
慢病毒和逆转录病毒载体技术的另一大进步是在动物和人体上的应用。 labome.cn | Another major step forward in lentiviral and retroviral vector technology has been their use in animals and humans. labome.com |
使用慢病毒或逆转录病毒系统可以使目标细胞基因组获得稳定可遗传的特定核酸序列。 labome.cn | Using lentiviral or retroviral [...] systems allows for the stable, heritable integration of a specific nucleic acid sequence [...]into the target cell’s genome. labome.com |
尽管慢病毒和逆转录病毒相对而言比其他病毒载体系统能容纳更大的插入序列，但包装还是有限度的。 labome.cn | While lentiviral and retroviral vectors can accommodate relatively large inserts as compared to other viral vector systems, [...] there is still a packaging limit. labome.com |
慢病毒和逆转录病毒基因递送系统利用了逆转录病毒复制方面的性质以获得目标核酸序列稳定的整合。 labome.cn | Lentiviral and retroviral [...] gene delivery systems exploit aspects of retrovirus replication to provide stable integration [...]of the desired nucleic acid sequence. labome.com |
他们有几种慢病毒载体 用于生产Lumio或V5表位标记的蛋白。 labome.cn | They include several lentiviral vectors for the [...] creation of Lumio or V5 epitope tagged proteins. labome.com |
尽管很多慢病毒载体 系统基于转运两个辅助质粒（第二代）和转运质粒，一些更新的系统（第三代）则将包装和包膜构建在三个质粒上再与转运质粒结合。 labome.cn | While many lentiviral vector systems [...] are based on transduction of two helper plasmids (second generation) with the transfer [...]plasmid, some newer systems (third generation) have the packaging and envelope constructs on three plasmids which are combined with the transfer plasmid. labome.com |
病毒被珠子俘获，然后失活，读出吸光度并与标准曲线比较（试剂盒提 供 慢病毒 的 RNA标准曲线）以确定核酸的含量。 labome.cn | The virus is captured by beads and then denatured, [...] and absorbance is read and compared to a [...] standard curve (Lentivirus RNA Standard [...]provided with the kit) to determine nucleic acid content. labome.com |
因为慢病毒在32 °C下比37 °C下更稳定，可以将包装细胞在此温度下培养直至收获病毒。 labome.cn | Since lentiviruses are more stable [...] at 32 °C than 37 °C, the packaging cells can be incubated at this temperature until the virus is collected. labome.com |
操作这些载体时主要的隐患在于(a)产生能自我复制的有感染能力 的 慢病毒 ； (b )肿瘤生成的潜在可能性；(c)转基因的潜在毒性。 labome.cn | The major concerns when working with these vectors are (a) the generation of replication-competent, contaminating lentivirus; (b) the potential for oncogenesis; and (c) the potential toxicity of the transgene. labome.com |
另外还有生产上的挑战，如保证纯度和高产，这是对临床级 的 慢病毒 载 体 的要求。 labome.cn | There are also manufacturing challenges, such as ensuring the purity and high yield required [...] for clinical grade lentiviral vectors. labome.com |
这种试剂盒能在45-60分钟内检测纯化 的 慢病毒 或 未纯化的上清液中的病毒核酸含量。 labome.cn | This measures the viral nucleic acid content of either purified lentivirus or unpurified supernatant in 45-60 minutes. labome.com |
在新一代的慢病毒系统 中，Tat已被剔除，Rev被置于一个单独的载体上以增加安全性。 labome.cn | Tat has been removed in newer [...] generations of lentiviral systems and Rev [...]has been placed on a separate vector to increase safety. labome.com |
这些慢病毒载体 在宿主细胞的细胞核内产生双链的游离DNA环。 labome.cn | These lentiviral vectors produce [...] double stranded episomal DNA circles in the host cell nucleus. labome.com |
第二和第三代慢病毒系统作为主要的可获得的商品化 的 慢病毒 系 统 采取了很多安全措施降低了这些风险 [17] 。 labome.cn | The second and third generation lentiviral systems, which make up most commercially available lentiviral systems, have many safety features that mitigate these risks [17] . labome.com |
核酸转染只能获得瞬时的转基因表达，而基于逆转 录 病毒 和 慢病毒 的 系 统却可以获得外源基因稳定的整合，外源基因于是被遗传下去随重复的细胞分裂持续表达。 labome.cn | Whereas transfection of nucleic acids results only in transient [...] transgene expression, the [...] activity of the viral integrase in retroviral and lentiviral based systems [...]allows for stable integration [...]of the transgene which is then inherited and continuously expressed over repeated cell divisions. labome.com |
近来，几个实验室都构造出了不通过传统的逆转录机制进行整合或者根本无需整合 的 慢病毒。 labome.cn | Recently, several labs have produced lentiviruses that do not integrate by the [...] traditional retrovirus mechanism, [...]or alternatively do not integrate at all. labome.com |
医生通常将脂肪肝与过量饮酒或慢性 病毒 性 肝炎联系在一起。 shanghai.ufh.com.cn | Physicians had usually associated fatty liver with [...] excessive drinking or chronic viral hepatitis. beijing.ufh.com.cn |
SBI提供基于HIV和FIV的慢病毒载体 ，其载体有很多启动子，取决于要转导的细胞类型：CMV（巨细胞病毒）用于HeLa及其他多种细胞；HEK293和HT1080转导；MSCV（小鼠干细胞病毒）用于造血细胞和干细胞转导；UbC（泛素C）用于转导大部分类型的细胞；PGK（磷酸甘油酸激酶）用于转导大部分类型的细胞；EF1（延伸因子1α）用于转导大部分类型的细胞。 labome.cn | SBI offers lentiviral vectors that are HIV- and FIV-based and have many promoters, depending on the cell type being transudced: CMV (Cytomegalovirus) for HeLa and many other cell types and for HEK293 and HT1080 transduction; MSCV (Murine Stem Cell Virus), for hematopoietic and stem cell transduction; UbC (Ubiquitin C), for transducing most cell types; PGK (Phosphoglycerate Kinase), for transducing most cell types; and EF1 (Elongation Factor 1α), for transducing most cell typesIn addition to the transfer vectors listed below, they offer inducible and dual promoter backbones. labome.com |
全球结核发病率在 2004 年似乎达到高峰，目前在世界大部分地区 缓 慢 下降 (艾滋病毒感染 率较高的非洲国家除外)。 daccess-ods.un.org | The global incidence of tuberculosis appears to [...] have peaked in 2004, [...] and is now falling slowly in most parts of the world (except in African countries with a high prevalence of HIV). daccess-ods.un.org |
即使他們感覺身 體健康，但病毒可能正在慢 慢地損壞他們的肝臟。 hepbsmart.com | Even though they [...] feel healthy, the virus may be slowly damaging their liver. hepbsmart.com |
我们现在可以把艾滋病毒作为一种慢 性 病 来 治 疗。 daccess-ods.un.org | We have also achieved universal access to HIV [...] treatment and we can now treat HIV as a chronic disease. daccess-ods.un.org |
我们深感关切地注意到癌症、心血管 疾 病、 慢性肾病、同毒性和 食物链中的农药相关联的疾病， 以及一些地区同空气质量有关的新的过敏疾病等的 出现。 daccess-ods.un.org | We note with deep concern the emergence of cancers, [...] cardiovascular disease, chronic kidney diseases, those linked to the toxicity and of pesticides [...]in the food chain, [...]and new allergic illnesses in some regions related to air quality, to mention only a few. daccess-ods.un.org |
Alios拥有一支由经验丰富的科学家和临床研究人员组成的创新团队，他们正在针对几种对公共健康至关重要的人类病毒病原体（包括肝 性 病毒 和 呼吸 道 病毒 ） 以 及其 他 慢 性 、急性和新出现 的 病毒 性 疾 病开发直接作用的抗病毒药。 tipschina.gov.cn | Alios has an innovative team of highly experienced scientists and clinical researchers who are developing direct acting [...] antiviral agents [...] against several human viral pathogens of public health importance including hepatotropic and respiratory viruses, and other chronic, acute and [...]emerging viral diseases. tipschina.gov.cn |
预防和战胜艾滋病毒/ 艾滋病、慢性病 和非传染疾病，加强疾病跟踪制度，有效地应 对现有疾病的传播和新疾病的出现。 daccess-ods.un.org | Prevent and combat HIV/AIDS, chronic and non-communicable diseases, and strengthen the disease follow-up system in order to effectively respond to the spread of existing diseases and the emergence of new varieties of those diseases. daccess-ods.un.org |
值得注意的是，本办事处在教育领域开展的计划主要关注以下战略支助领域，所有这 些都直接回应肯尼亚教育部门支助计划（KESSP）所载的战略和成果，教育部门计划实现 《2030 年展望》：通过教育管理信息系统加强规划、监测和评估教育系统；加强教育部门 以应对艾滋病毒和艾 滋病；提高教育促和平计划的质量；以及通过宣传和技术支持战略规划 来加强扫盲发展。 unesdoc.unesco.org | It is noteworthy that programmes implemented by the Office in the area education focused on the following strategic areas of support, all directly responding to strategies and results contained in Kenya Education Sector Support Programme (KESSP), the education sector’s plan to materialize Vision 2030: strengthening the planning, monitoring and evaluation of the education system through EMIS; enhancing the education sector responses to HIV and AIDS; improving the quality of education for peace programme; and strengthening literacy development through advocacy and technical support to strategic planning. unesdoc.unesco.org |
由於該病毒是具潛在危險的病原體，因此化驗室如出現 人類豬型甲型流行性感冒(H1亞型)病毒 逸 漏 事故，必須受法定呈 報規定所管制，以便及早偵察有關事故，並及時採取適當的控制 措施，以保障化驗室人員的健康和防止疾病蔓延。 legco.gov.hk | As the virus is a potentially dangerous pathogen, it is important for incidents of leakage of human swine influenza A virus (subtype H1) [...] from laboratories to [...]be subject to the statutory notification requirement, so as to facilitate early detection of such incidents, and to enable appropriate control measures to be implemented in a timely manner for the protection of laboratory workers and prevention of spread of diseases. legco.gov.hk |
英汉双解词典包含2273206条英汉词条，基本涵盖了全部常用单词的翻译及用法，是英语学习的有利工具。