| 单词 | 大肠杆菌 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
| 释义 | 大肠杆菌 —Escherichia coli (E. coli)See also:大肠—the large intestine 大肠 n—large intestine n 杆菌—bacillus (any rod-shaped bacteria)
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瓦克获有专利的大肠杆菌分泌 技术ESETEC®是一项可靠的生产蛋白质和抗体片段的低成本技术。 wacker.com | The patented ESETEC® secretion system from WACKER, [...] which is based on E. coli, is a tried-and-tested [...]technology for the cost-effective production [...]of proteins including antibody fragments. wacker.com |
大肠杆菌分泌菌株在发酵过程中非常稳定，并且可以直接使用容量达4 m³的发酵桶进行商业化生产。 wacker.com | The E. coli secretion strain is extremely [...] stable during fermentation and is routinely employed in commercial-scale production [...]using fermenters with capacities of up to 4 m³. wacker.com |
水体中的大肠杆 菌浓度 ，是衡量病原体污染的最常用指标。 unesdoc.unesco.org | For example, predation mortality is often highest on pre-recruits and models that explicitly consider the biological interactions in the stock-recruitment relationship are more realistic. unesdoc.unesco.org |
该系统以大肠杆菌 K12菌株和一系列高表达质粒为基础。 wacker.com | It is based on an E. coli K12 strain and a series of highly expressive plasmids. wacker.com |
大肠杆菌分泌 菌株能将天然构象的蛋白质穿过细胞外膜转移到发酵培养基中。 wacker.com | The E. coli secretion strain can transfer [...] proteins in native conformation across the outer cell membrane into the culture medium. wacker.com |
已有研究报告表明，同传统技术相比， 利用以大肠杆菌为基 础的ESETEC®技术生产的重组体产品， 产率有明显提高。 wacker.com | A study has shown that E. coli-based ESETEC® is capable of delivering much higher yields of the recombinant target product than conventional technologies. wacker.com |
DENSETEC® 是瓦克基于专利技术开发的一种高效 的 大肠杆菌 高 细 胞密度发酵技术品牌。 wacker.com | DENSETEC® is the trade name of a highly effective high-cell-density fermentation of E. coli, developed by WACKER and based on proprietary know-how. wacker.com |
通过将DENSETEC® 高细胞密度发酵技术与ESETEC® 大肠杆菌技术 相结合，加快了工艺开发的进度，简化了提纯程序，从而确保了经济高效的药用蛋白制造。 wacker.com | Combining the [...] DENSETEC® high-cell-density fermentation with the ESETEC® E. coli secretion technology [...]accelerates process [...]development and simplifies the purification procedure – and therewith ensures the efficient and cost-effective manufacture of pharma proteins. wacker.com |
除了控制细菌细胞的基因组的化学合成( 克莱格·温特尔人工生命) ，其他方 法包括：通过在酵母中制作新陈代谢途径生产抗疟疾药物的前体；合成哺乳动物 [...] 基因电路揭示抗结核化合物；广泛存在的生物计算的展示；以及制 作 大肠杆菌以 探测和杀死人体内病原体。 daccess-ods.un.org | In addition to the chemical synthesis of a genome able to control a bacterial cell (Craig Venter’s artificial life) other important stepping stones include: the engineering of the metabolic pathway in yeast to produce the precursor of an anti-malarial drug; the creation of a synthetic mammalian gene circuit that revealed anti-tuberculosis compounds; a [...] demonstration of distributed biological computation; and the [...] engineering of an E. coli to sense and kill [...]a human pathogen. daccess-ods.un.org |
两个实例是大肠杆菌分泌 系统ESETEC®和高细胞密度发酵技术 DENSETEC®。 wacker.com | Two examples [...] are its E. coli-based secretion technology ESETEC® and its high-cell-density [...]fermentation technology DENSETEC®. wacker.com |
Junhee Seok及其同事研究了创伤、烧伤和来自例 如 大肠杆菌 等 细 菌的毒素是如何影响病人的遗传应答的。 chinese.eurekalert.org | Junhee Seok and colleagues studied how trauma, burns, [...] and toxins from bacteria like E. coli affected [...]genetic responses in patients. chinese.eurekalert.org |
他指出最近发生的一系列食品安全突发 事件，诸如日本食品遭到放射性物质污染，德国暴发肠出血 性 大肠杆菌 疫 情，以及故 意使用邻苯二甲酸盐污染食品和饮料等事件均表明食品市场的全球化已经达到何种高 度，世界一个国家或一个地区发生的事件会多么迅速地影响到其它国家和地区。 codexalimentarius.org | He pointed out that recent food safety emergencies, such as the contamination of food with radioactive substances in Japan, the outbreak of EHEC in Germany, or the intentional contamination of food and beverages with phthalates had illustrated how globalised the food market has become and how rapidly incidents in one country or part of the world can impact others. codexalimentarius.org |
Pedro Kouri Institute 是拉丁美洲负责监测分子流行病学(沙门氏菌、Escheri qu ia 大 肠杆菌，痢疾和霍乱弧菌)网的 PULSENET 的成员。 daccess-ods.un.org | IPK is a member PULSENET, the Latin American network responsible for molecular epidemiological surveillance of salmonella, escheriquia coli, shigella and vibrio cholera. daccess-ods.un.org |
自从引进这一创 新技术以来， 越来越多生物药剂领域的企业采用了 大肠杆菌 ( E. coli)生 产系统， 希望以此缩短开发时间， 从中受益。 wacker.com | Since we introduced this innovation to the biopharmaceutical market we see that more and more companies revisit E. coli as production host to benefit from shorter development timelines”, he added. wacker.com |
ESETEC® 大肠杆菌分泌 技术是生产抗体片段的理想选择。 wacker.com | Product yields can already exceed 10 g/l. The ESETEC® E. coli secretion technology is ideal for antibody fragment production. wacker.com |
玉米rayado菲诺的病毒 [...] （MRFV）外壳蛋白的自组装生产稳定和空的病毒样颗粒是稳定的蛋白质-蛋白质 在 大肠杆菌 中 的 相互作用，这可用于在病毒为基础的技术的应用程序8。 jove.com | Maize rayado fino virus (MRFV) coat proteins [...] self-assemble in Escherichia coli producing stable [...]and empty VLPs that are stabilized by protein-protein [...]interactions and that can be used in virus-based technologies applications 8. jove.com |
在过去 100 多年里，饮用水、娱乐休闲水域和贝类收获区水域的卫生质量标准，都 是以测定大肠杆菌或排泄物大肠杆菌 等 排泄物指标生物的浓度为基础的。 unesdoc.unesco.org | Both systems require major investment to increase capacity for handling much larger volumes of more diverse data (biological, chemical and geological variables), greater distribution of model outputs, and rapid exchange of large volumes of data between data centres. unesdoc.unesco.org |
大约3年前，斯洛伐克的鸽子受到一次严重的沙门氏菌 和 大肠杆菌 的 感 染，因为卫生保健薄弱，压力大，迫使PHARMAGAL BIO开始做医药研究，并发明了几种疫苗，它们对预防巴拉米哥 ， 大肠杆菌 ， 和 疱疹病毒等很有效。 herbots.be | Since there [...] was a strong infection of pigeons in Slovakia some three years ago that strongly resembled Salmonella and EColli, due to weak hygiene and high stress,it was necessary for PHARMAGAL BIO to start research. herbots.be |
我们之前的“当班出结果”的 大肠杆菌 0 1 5 7检 测试样（包括增菌阶段）和现在的“当天出结果”的李斯特菌属某些种检测试样均获得了AOAC PTM认证，为FoodChek向全球食品生产商推广其专利的MICT纳米技术检测平台打开了大门。 tipschina.gov.cn | Securing the AOAC PTM Certification, previously with our "same shift" E. coli O157 assay (including enrichment phase), and now with the "same day" Listeria spp. assay opens the door for FoodChek to introduce its patented MICT nanotechnology testing platform to all processors worldwide. tipschina.gov.cn |
食 物 中 的 总 含 菌 量 及 大 肠 杆 菌 含 量 ( 总 数 ) 能 反 映 食 物 的 卫 生 品 质 。 cfs.gov.hk | The hygienic qualityof food is reflected [...] in the total bacterial count (TBC) and total E. coli(total) count. cfs.gov.hk |
α-银环蛇毒素在神经科学研究以及在临床和制药上有重要作用，为获得大量的α-银环蛇毒素，我们用已构建的pGEX-BgTX（P22-A31）质粒 在 大肠杆菌 B L 2 1（ DE3）中表达，并用谷胱甘肽Sepharose [...] FF纯化GST-α-银环蛇毒素融合蛋白，再用凝血酶切掉融合标签谷胱苷肽转移酶（Glutathione [...]S-transferase，GST），得到了较纯的重组α-银环蛇毒素同工毒素，得率约为1.225 mg/L。 actazool.org | In order to acquire quantitites of α-bungarotoxin, we expressed [...] GST-α-bungarotoxin fusion protein using [...] constructed plasmid pGEX-BgTX (P22-A31) in E.coli BL21 (DE3)cell, [...]purified GST-α-bungarotoxin [...]using glutathione-Sepharose FF, cleavaged Glutathione S-transferase(GST) tag by thrombin in the column, and finally, we obtained recombinant α-bungarotoxin with yields of about 1.225 mg/L. In order to compare the immunogenicity of recombinant protein and crude α-bungarotoxin, we prepared multi-antibody using recombinant α-bungarotoxin. actazool.org |
为研究促甲状腺激素释放激素(thyrotrophin-releasing hormone,TRH)及其受体 (TRH receptor, TRH-R)在大鼠睾丸组织中的表达规律和在生殖发育调节中的作用，依据大鼠下丘脑中的前TRH原(preproTRH, ppTRH)和垂体中的TRH-R cDNA设计引物，采用RT-PCR法从大鼠睾丸组织中获得了ppTRH和TRH-R的cDNA克隆，测序后构建表达载体， 在 大肠杆菌 中 表达了可溶性的ppTRH和TRH-R融合蛋白。 actazool.org | In order to investigate the expression regulation of thyrotrophin-releasing hormone (TRH) and TRH receptor (TRH-R) in rat testis, and to study their function in reproduction and development, we designed oligonucleotide primers from the sequences of rat hypothalamus prepro(pp)TRH and pituitary TRH-R cDNA for reverse transcription-polymerase chain reaction (RT-PCR). actazool.org |
ESETEC® E. coli（大肠杆菌）分 泌技术能将天然构象的蛋白质穿过细胞外膜转移到培养基中，从而实现了更加高效和低成本的药用蛋白生产。 wacker.com | ESETEC® E. coli secretion technology can transfer proteins in their native conformation across the outer cell membrane into the culture medium, enabling more efficient and cost-effective production of pharmaceutical proteins. wacker.com |
按照人金属硫蛋白-3(hMT-3)的基因序列,选 用 大肠杆菌 偏 爱的密码子合成了全长hMT-3基因,并将其插 入 大肠杆菌 融 合 表达质粒pALEX的多克隆位点中,在谷胱甘肽-硫-转移酶(GST)下游与GST融合表达。 chrom-china.com | The gene encoding human metallothionein-3 (hMT-3) was synthesized and inserted into the poly-cloning sites of fusion expression vector pALEX, and fused downstream to its glutathione S-transferase (GST) fusion partner. chrom-china.com |
ESETEC®技术以大肠杆菌K12菌株为基础， 这种菌株能够在发酵过程中使重组体蛋白质以正确的折叠结构移入培养基， 这样， 目标蛋白质的提纯因为可以省去复杂的同质化和折叠构象恢复工序而得到简化， 生产及成本的总体效益明显提高。 wacker.com | It is based on an E. coli K12 strain which has a particular ability to secrete recombinant proteins into the culture broth in the correct folding conformation. wacker.com |
一 九 九 七 至 一 九 九 九 年 间 ， 先 后 发 现 有 82 个 三 文 治 样 本 的 总 含 菌 量 达 不 满 意 水 平 ， 而 所 有 样 本 的 大 肠 杆 菌 含 量 ( 总 数 ) 均 达 满 意 水 平 。 cfs.gov.hk | From 1997 through 1999, 82 samples of sandwiches had unsatisfactorylevel of TBC and no sample was found unsatisfactory in E. coli (total)count. cfs.gov.hk |
本研究用DNA重组技术将中华绒螯蟹（Eriocheir japonica sinensis）的蜕皮抑制激素1 [...] （Ers-MIH1）成熟肽的cDNA序列亚克隆至原核表达载体pET-28a(+)中，并 在 大肠杆菌 B L 21 (DE3)中进行高效表达。 actazool.org | The cDNA encoding the molt-inhibiting hormone 1 (Ers-MIH1) of the mitten crab Eriocheir japonica sinensis was inserted into pET28a(+) bacterial expression vector by [...] using recombinant DNA techniques and the fusion [...] protein was highly expressed in inclusion bodies in E. coli BL21 (DE3). actazool.org |
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