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| 释义 |
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把长好的小鼠胚胎成纤维细胞的培养基吸掉，加入10ml新鲜的MEF培养基，并在避光的条件下加入110μl配好的丝裂霉素C，混匀后放入培养箱培养2h。 labome.cn | Aspirate the MEF medium, add 10ml fresh MEF [...] mediumwith 110μl mitomycin C solution [...]in dark, incubating the cells in the [...]carbon dioxide incubator with 37℃, 5% CO2 after mixing gently for 2 hours. labome.com |
对于每个剩余的井，再悬浮CALU-3细胞中加入100μl，轻轻垂钓吸移管对内部插入壁的引导通道，并慢慢地释放细胞。 jove.com | To each of the [...] remaining wells,add100μl of resuspended Calu-3 [...]cells, gently angling the pipette against the interior [...]guide channel of the insert wall and slowly releasing the cells. jove.com |
对于未感染的井，添加100μl的无血清的EMEM根尖室中适当的Transwell小室内，用于模拟感染的井，加入100μl的无病毒的制剂稀释于无血清的EMEM心尖室适当的Transwell小室内，并病毒感染，稀释在无血清的EMEM病毒和根尖的室中适当的Transwell小室内加入100μl。 jove.com | For uninfected wells, add100 μlof serum-free EMEM to the apical compartment of appropriate Transwells, for mock-infected wells, add 100 μlof virus-free preparation diluted in serum-free EMEM to the apical compartment of appropriate Transwells, and for virus infection, dilute virus in serum-free EMEM and add 100 μlto the apical compartments [...] of appropriate Transwells. jove.com |
1）优化的检测条件包括采用2μl血清与3×104计数·min-1的标记抗原缓慢振荡保温24 [...] h，采用4℃PBS缓冲液洗涤沉淀物10次，采用Optiphase Supermix型闪烁液测量放射性计数。 zhhyx.com.cn | Results (1)The optimized conditions of [...] micro-plate RBAincluded 2 μlserumincubated with [...]3×104 counts/min 35S-GAD for 24 h under [...]slow vibration, antigen-antibody compounds washed 10 times by 4℃ PBS buFer, and radioactivity counted with Optiphase Supermix scintillation liquid. zhhyx.com.cn |
在基线CD4+细胞计数小等于50 个细胞/μL时，ART中位延迟从实施之前的5.8 周（四分位距，IQR：3.7-9.0）下降到实施之后的3.0 周（IQR：2.1-4.4）（P <0.001）；在基线CD4+细胞计数大于50个细胞/μL时，延迟从7.0 周（IQR：5.3-11.3）下降到3.6 周（IQR：2.9-5.3）（P <0.001）。 who.int | With baseline CD4+ cell [...] counts ≤ 50 cells/µL, median delay to ART declined from 5.8 weeks (interquartile range, IQR: 3.7–9.0) before to 3.0 weeks (IQR: 2.1–4.4) after implementation (P < 0.001); with baseline CD4+ cell counts > 50 cells/µL, delay dropped from [...]7.0 (IQR: 5.3–11.3) [...]to 3.6 (IQR: 2.9–5.3) weeks (P < 0.001). who.int |
用250μL二次抗体孵育的细胞在室温下1小时，保持避光（我们用羊抗小鼠IgM Cy3标记共轭，羊抗鼠IgG [...] Cy3标记山羊抗大鼠IgM抗体Cy3标记共轭轭，和山羊抗兔Cy3标记物）在1:300稀释。 jove.com | Incubate the cells with 250μl of secondary antibody [...] for 1 hour at room temperature, keeping away from light (we used Goat [...]anti-Mouse IgM Cy3 conjugate, Goat anti-Mouse IgG Cy3 conjugate, Goat anti-Rat IgM Cy3 conjugate and Goat anti-Rabbit Cy3 conjugate at 1:300 dilutions). jove.com |
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