单词 | pptrh | ||||||
释义 |
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这些结果表明: 大鼠睾丸组织能特异性表达ppTRH和TRH-R,并且表达量与发育过程相关。 actazool.org | These Results suggest that rat testis specifically express TRH and TRH-R, and the transcription of ppTRH and TRH-R gene in the rat testis is development-dependent. actazool.org |
ppTRH和TRH-R体外表达产物的获得为后续研究其功能奠定了基础。 actazool.org | The acquirement of expressed products for ppTRH and TRH-Rcan be used for further research on physiological significance of TRH and TRH-R expression in rat testis. actazool.org |
为研究促甲状腺激素释放激素(thyrotrophin-releasing hormone,TRH)及其受体 (TRH receptor, TRH-R)在大鼠睾丸组织中的表达规律和在生殖发育调节中的作用,依据大鼠下丘脑中的前TRH原(preproTRH,ppTRH)和垂体中的TRH-R cDNA设计引物,采用RT-PCR法从大鼠睾丸组织中获得了ppTRH和TRH-R的cDNA克隆,测序后构建表达载体,在大肠杆菌中表达了可溶性的ppTRH和TRH-R融合蛋白。 actazool.org | In order to investigate the expression regulation of thyrotrophin-releasing hormone (TRH) and TRHreceptor (TRH-R) in rat testis, and to study their function in reproduction and development, we designed oligonucleotide primers [...] from the sequences of rat hypothalamus [...]prepro(pp)TRH and pituitary TRH-R cDNA for reverse transcription-polymerase chain reaction (RT-PCR). actazool.org |
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