Molecular-weight size marker

Electrophoresed gel with DNA ladders of varying lengths in left lane and middle lane. Fragments sizes are marked on the right, in base pairs.

1.2% agarose gel showing two different DNA ladders dyed with GelRed stain

Immunoblot with protein ladder in leftmost lane. Fragment sizes are measured in kDA (kilodaltons).

A molecular-weight size marker, also referred to as a protein ladder, DNA ladder, or RNA ladder, is a set of standards that are used to identify the approximate size of a molecule run on a gel during electrophoresis, using the principle that molecular weight is inversely proportional to migration rate through a gel matrix. Therefore, when used in gel electrophoresis, markers effectively provide a logarithmic scale by which to estimate the size of the other fragments (providing the fragment sizes of the marker are known).

单词	DNA ladder
释义	DNA ladder 英语百科 Molecular-weight size marker A molecular-weight size marker, also referred to as a protein ladder, DNA ladder, or RNA ladder, is a set of standards that are used to identify the approximate size of a molecule run on a gel during electrophoresis, using the principle that molecular weight is inversely proportional to migration rate through a gel matrix. Therefore, when used in gel electrophoresis, markers effectively provide a logarithmic scale by which to estimate the size of the other fragments (providing the fragment sizes of the marker are known).
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