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单词 Displacement Chromatography
释义

Displacement Chromatography

英语百科

Displacement chromatography

Example of a Langmuir isotherm, for a population of binding sites having uniform affinity. In this case the vertical axis represents the amount bound per unit of stationary phase, the horizontal axis the concentration in the mobile phase. In this case, the dissociation constant is 0.5 and the capacity 10; units are arbitrary.

Displacement chromatography is a chromatography technique in which a sample is placed onto the head of the column and is then displaced by a solute that is more strongly sorbed than the components of the original mixture. The result is that the components are resolved into consecutive “rectangular” zones of highly concentrated pure substances rather than solvent-separated “peaks”. It is primarily a preparative technique; higher product concentration, higher purity, and increased throughput may be obtained compared to other modes of chromatography.

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