纸色谱法（又称纸色层分析法，英文：Paper chromatography）是分析化学中一种用来分离混合物的色谱技术。纸色谱法主要是用来分析染料，它已经在很大的程度上被薄层色谱法取代，但仍然是一种很好的教学工具。 当初发展纸色谱法是为了分离植物的色素制成颜料，用此方法可以分离出不同颜色的色素，所以这个技术有一个英文名称chroma就是拉丁文「颜色」的意思。不过无色的混合物也可用这种方法分离，只要各成分对于溶剂及固定相有不同的亲和力就行了。 双向纸上色层分析法也是纸色谱法的一种，先使用一种溶剂，进行色层分析后将试纸旋转90度，再用另一种溶剂进行色层分析，常用在类似化合物（如胺基酸）组成混合物的分析。

Paper chromatography is an analytical method that is used to separate coloured chemicals or substances.This can also be used in secondary or primary colours in ink experiments. This method has been largely replaced by thin layer chromatography, but is still a powerful teaching tool. Double-way paper chromatography, also called two-dimensional chromatography, involves using two solvents and rotating the paper 90° in between. This is useful for separating complex mixtures of compounds having similar polarity, for example, amino acids. If a filter paper is used, it should be of a high quality paper. The mobile phase is developing solutions that can travel up to the stationary phase carrying the sample along with it.