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单词 Chemical sequencing
释义

Chemical sequencing

英语百科

Maxam–Gilbert sequencing

An example Maxam–Gilbert sequencing reaction. Cleaving the same tagged segment of DNA at different points yields tagged fragments of different sizes. The fragments may then be separated by gel electrophoresis.

Maxam–Gilbert sequencing is a method of DNA sequencing developed by Allan Maxam and Walter Gilbert in 1976–1977. This method is based on nucleobase-specific partial chemical modification of DNA and subsequent cleavage of the DNA backbone at sites adjacent to the modified nucleotides.

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