翼手龙亚目（学名：Pterodactyloidea），希腊文意思为“有翼的手指”，是翼龙目两个亚目之一，包括翼龙类的较先进物种。牠们在中侏㑩纪出现，与喙嘴翼龙亚目的差别在于牠们的短尾巴，与翼上的长指骨。最先进的物种缺乏牙齿。许多物种头颅上有发展良好的冠饰，这是一种展示物，夜翼龙与古神翼龙拥有巨大的冠饰。翼手龙类，尤其是神龙翼龙科，是白垩纪末最后存活的翼龙目动物。白垩纪-第三纪灭绝事件灭绝了翼龙类、恐龙、与大多数海生爬行动物。

目前已知最早的翼手龙类化石，发现于英国的Taynton石灰岩组地层，地质年代约1亿6600万年前，相当于侏㑩纪中期的巴通阶。这些标本极可能属于梳颌翼龙超科。

Pterodactyloidea (derived from the Greek words πτερόν (pterón, for usual ptéryx) "wing", and δάκτυλος (dáctylos) "finger" meaning "winged finger", "wing-finger" or "finger-wing") is one of the two traditional suborders of pterosaurs ("wing lizards"), and contains the most derived members of this group of flying reptiles. They appeared during the middle Jurassic Period, and differ from the basal (though paraphyletic) rhamphorhynchoidea by their short tails and long wing metacarpals (hand bones). The most advanced forms also lack teeth. Many species had well developed crests on the skull, a form of display taken to extremes in giant-crested forms like Nyctosaurus and Tupandactylus. Pterodactyloids (specifically the family Azhdarchidae) were the last surviving pterosaurs when the order became extinct at the end of the Cretaceous Period, together with the non-avian dinosaurs and most marine reptiles.