Strong expression is first observed in prechordal plate at the stage of 50% epiboly, l the stained cells then migrate to the proctodeum.
整胚原位杂交结果显示该基因最早主要在50%外包期的前脊索盘中表达,既而迁移到原肛处。
英语百科
Prechordal plate
In the development of vertebrate animals, the prechordal plate is a "uniquely thickened portion" of the endoderm that is in contact with ectoderm immediately rostral to the cephalic tip of the notochord. It is the most likely origin of the rostral cranial mesoderm.