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单词 PNPP
释义

PNPP

英语百科

para-Nitrophenylphosphate

Skeletal formula of para-nitrophenylphosphate
Space-filling model of the para-nitrophenylphosphate molecule

para-Nitrophenylphosphate (pNPP) is a chromogenic substrate for acid and alkaline phosphatase in ELISA and conventional spectrophotometric assays. These enzymes catalyse the hydrolysis of pNPP to yellow para-nitrophenol. This product absorbs strongly at 405 nm and can be measured with a visible light spectrophotometer.

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