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单词 PCR amplification
释义

PCR amplification

中文百科

聚合酶链式反应 Polymerase chain reaction

(重定向自PCR amplification)
PCR所配的热循环设备
热循环机

聚合酶连锁反应(英文:Polymerase chain reaction,缩写:PCR),是一种分子生物学技术,用于扩增特定的DNA片段,这种方法可在生物体外进行,不必依赖大肠杆菌或酵母菌等生物体。聚合酶链式反应技术被广泛地运用在医学和生物学的实验室,例如用于判断检体中是否会表现某遗传疾病的图谱、传染病的诊断、基因复制,以及亲子鉴定。

英语百科

Polymerase chain reaction 聚合酶链式反应

(重定向自PCR amplification)
A strip of eight PCR tubes, each containing a 100 μl reaction mixture
Placing a strip of eight PCR tubes into a thermal cycler
A thermal cycler for PCR
An older model three-temperature thermal cycler for PCR

The polymerase chain reaction (PCR) is a process used in molecular biology to amplify a single copy or a few copies of a piece of DNA across several orders of magnitude, generating thousands to millions of copies of a particular DNA sequence.

Developed in 1983 by Kary Mullis, PCR is now a common and often indispensable technique used in medical and biological research labs for a variety of applications. These include DNA cloning for sequencing, DNA-based phylogeny, or functional analysis of genes; the diagnosis of hereditary diseases; the identification of genetic fingerprints (used in forensic sciences and DNA paternity testing); and the detection and diagnosis of infectious diseases. In 1993, Mullis was awarded the Nobel Prize in Chemistry along with Michael Smith for his work on PCR.

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