美莱村屠杀（越南语：Thảm sát Mỹ Lai／惨杀美莱）是越战期间，美军第23步兵师第11旅第20团第1营C连的官兵，于1968年3月16日在越南广义省的美莱村（My Lai）进行的屠杀。男女老幼甚至婴儿都被杀害，亦有女性被轮奸和尸体被肢解。

屠杀事件被掩盖了一年多，直到先后几个美国士兵写信反映自己所在部队的暴行，并提到这个惨绝人寰的大屠杀。这件惨案推动美国国内的反战情绪。至于遇难者人数，不同的资料来源对于美莱村越南平民的实际死亡数目也各不相同。美**方的官方报告指称168人死亡，其中20%是越南平民；越南的官方报告则称住在村里的900名平民中，有568名被杀害。而《纽约时报》的一条消息指出，双方政府私下同意，死亡数目在400人到500人之间。

The Mỹ Lai Massacre (Vietnamese:thảm sát Mỹ Lai [tʰɐ̃ːm ʂɐ̌ːt mǐˀ lɐːj], [mǐˀlɐːj]; /ˌmiːˈlaɪ/, /ˌmiːˈleɪ/, or /ˌmaɪˈlaɪ/) was the Vietnam War mass killing of between 347 and 504 unarmed civilians in South Vietnam on March 16, 1968. It was committed by U.S. Army soldiers from the Company C of the 1st Battalion, 20th Infantry Regiment, 11th Brigade of the 23rd (Americal) Infantry Division. Victims included men, women, children, and infants. Some of the women were gang-raped and their bodies mutilated. Twenty-six soldiers were charged with criminal offenses, but only Lieutenant William Calley Jr., a platoon leader in C Company, was convicted. Found guilty of killing 22 villagers, he was originally given a life sentence, but served only three and a half years under house arrest.