Hopkins-Cole reaction

The Hopkins-Cole reaction, also known as the glyoxylic acid reaction, is a chemical test used for detecting the presence of tryptophan in proteins. A protein solution is mixed with Hopkins Cole reagent, which consists of glyoxylic acid. Concentrated sulfuric acid is slowly added to form two layers. A purple ring appears between the two layers if the test is positive for tryptophan. Nitrites, chlorates, nitrates and excess chlorides prevent the reaction from occurring.

单词	Glyoxylic acid reaction
释义	Glyoxylic acid reaction 英语百科 Hopkins-Cole reaction The Hopkins-Cole reaction, also known as the glyoxylic acid reaction, is a chemical test used for detecting the presence of tryptophan in proteins. A protein solution is mixed with Hopkins Cole reagent, which consists of glyoxylic acid. Concentrated sulfuric acid is slowly added to form two layers. A purple ring appears between the two layers if the test is positive for tryptophan. Nitrites, chlorates, nitrates and excess chlorides prevent the reaction from occurring.
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Glyoxylic acid reaction

Hopkins-Cole reaction