Gambolò is a comune (municipality) in the Province of Pavia in the Italian region Lombardy, located about 35 kilometres (22 miles) southwest of Milan and about 25 km (16 mi) northwest of Pavia.

Gambolò borders the following municipalities: Borgo San Siro, Mortara, Tromello, Vigevano. Sights include the Litta Castle, the parish church of Sts. Eusebius and Gaudentius (in neo-medieval style) and the Pieve of Sant'Eusebio.