In vitro culture system of buffalo fetal fibroblasts ( BFF ) was investigated in this study.

本实验探讨了适合水牛胎儿成纤维细胞 ( BFF ) 体外培养的体系.

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Comparison of 10 % bFF and 10 % FBS with oocytes maturation; Efficiency of micro - drop culture was assessed.

对比 10%bFF 和10%胎牛血清(FBS),以及观察微滴培养对卵母细胞成熟的影响.

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Therefore adding 10 % of mixed BFF in maturation medium is the best choice.

因此,成熟培养液中添加10%的混合BFF较为适宜.

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Therefore, adding 10 % of mixing BFF in maturation medium could be the best choice.

因此, 成熟培养液中以添加10%的混合BFF较为适宜.

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Among the three groups adding BFF at 10 % concentrationexpressed the best proliferous effect.

细胞的表型,以添加10%BFF的培养液促增殖效果最好.

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The fertilization inhibitory factors of BFF may be derived from serum rather than from follicular cells.

BFF的这种受精抑制因子可能来自血清而不是卵泡细胞.

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Shoulder pads may come and go, but a BFF is forever.

垫肩的流行趋势来了又走, 但好朋友是永远的.

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In addition , the addition of 20 % bFF in maturation medium inhibited the hatching of blastocyst.

添加20%卵泡液的培养液对囊胚的孵化有抑制作用.

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Along with the increase of bFF, in IVF group , cleavage and blastocyst rate decreases.

添加D0血清体外受精胚胎卵裂率较高, 但对囊胚发育率影响差异不显著(P>0.05).

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