RT - PCR was used to determine GK and PEPCKgene expression ( corrected by ? ? - actin ) .

用RT -PCR 检测GK和 PEPCK mRNA表达水平的变化.

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Methods Polymerase chain reaction ( PCR ) connected with reverse dot blot ( RDB ) were performed.

方法采用多聚酶链反应 ( PCR ) 结合反向斑点杂交 ( RDB ) 技术.

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The mutation was confirmed by allele specific PCR ( ASPCR ).

应用等位基因特异性 PCR 验证测序所发现的突变.

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Methods PCR was performed to amplify the AMG encoding region.

方法　采用PCR技术体外扩增AMG完整分泌肽编码区.

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Results CnA α, CnA ? ? could be detected by RT - PCR but no CnA ? ?.

结果RT -PCR 可检测到CnAα 、 CnAβ,未检测到CnAγ.

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Histamine - producing strains could be detected by PCR test or DNA probe technology.

利用PCR与DNA探针技术能够快速检测葡萄酒中的组胺产生菌.

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RT - PCR was used to detect the eNOS mRNA and iNOS mRNA.

RT -PCR 分别检测内皮细胞eNOS、iNOS基因的相对表达量.

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The ISOObp promoter of PNZIP gene was cloned by adaptor PCR method.

因此,我们利用接头PCR技术克隆了PNZIP基因的启动子.

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PCR were using to detect bla ( TEM ), bla ( SHV ) and bla ( CTX - M ) genes.

应用聚合酶链反应(PCR)方法扩增产ESBLs株的bla ( TEM ) 、 bla ( SHV ) 和bla ( CTXM ) 基因.

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Results The structures of recombinant plasmids were comfinaed by PCR and sequencing.

结果重组体经PCR和测序证实准确连接.

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The expressions of bcl - x, bax and eNOS were measured by SQ - RT - PCR technique.

采用半定量逆转录聚合酶链反应(SQ-RT-PCR)法检测内皮细胞bcl-x 、 bax以及 eNOS表达.

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EST - PCR and EST - SSR marker were developed in Chinese cabbage.

建立了白菜的EST -PCR 和EST-SSR标记.

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Expression of TGF ? ? 1 mRNA was evaluated by RT PCR.

RT?PCR方法测定肺组织内TGF?β1的表达.

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REP - PCR , AFLP technique can reveal the genetic differentiation properly.

AFLP和 REP -PCR 技术均能很好地反映菌株的遗传学差异.

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Methods: Polymer - ase chain reaction ( PCR ) was used to determine the ACE genotype.

方法: 采用聚合酶链反应 ( PCR ) 方法检测ACE基因型.

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