Methods Polymerase chain reaction ( PCR ) connected with reverse dot blot ( RDB ) were performed.

方法采用多聚酶链反应 ( PCR ) 结合反向斑点杂交 ( RDB ) 技术.

互联网

Conclusion The established dot blot technique is practicable and economical.

结论建立的斑点杂交技术经济简便,切实可行.

互联网

The result of rt - pcr was similar to reverse dot - blot.

其升高趋势与反向点杂交结果相同.

互联网

Results We established and optimized the dot blot technique which had reliable results and low background.

结果建立并优化了膜斑点杂交的条件,本底清晰,结果可靠.

互联网

The similar result of RT - PCR and reverse dot - blot high reliability of reverse dot - blot.

同时也反应了反向多点杂交结果的可靠性.

互联网

Two probing strategies hae generally been employed, namely, dot - blot hybridization and fluorescent in situ hybridization.

一般有两种探针策略可以使用, 即点杂交和荧光原位杂交.

互联网