GFP

GFP has also been used for more, um, creative purposes.

In one of his first experiments, he coloured six individual cells in the transparent roundworm Caenorhabditis elegans with the aid of GFP.

Note that you use your previously initialized variables along with a fourth parameter that indicates the GFP mask for memory initialization.

The natural fluorescence of GFP is incoherent, just like the light emitted by a normal light bulb.

The intense fluorescence of GFP is due to the nature of a chromophore composed of modified amino acids within the polypeptide.

Gather and Yun put human embryonic kidney cells into a Petri dish and then added the DNA that encodes for GFP to the cells.

A tantalizing prospect because almost any organism, from a bacterium to a cow, can be programmed to synthesize GFP.

If researchers can harvest something good from all that extra GFP in the water, more power to them.

GFP, as it is known to its friends, gives the glow to Aequorea victoria, a jellyfish that lives in the eastern Pacific Ocean.

Results A GFP mouse tumor model and a GFP mouse bearing DsRed tumor mouse model were successfully established.

We present a patient case relating to a rare benign tumor of the hypopharynx (giant fibrovascular polyp, GFP).

Osamu Shimomura first isolated GFP from the jellyfish Aequorea victoria, which drifts with the currents off the west coast of North America.

The variant GFP encoded by the synthetic gene can be expressed at a high level, forming approximately 5% of total leaf protein.

Dr Tsien's contribution was to tinker with the GFP gene, and the genes of related proteins found in corals, to produce other colours.

The article on the physical and chemical properties, the fluorescence characteristics, improvement and application of GFP are reviewed.

Expression of GFP was evaluated by flow cytometry, and the best MOI would be calculated.

When exposed to the blue light from the bioluminescent reaction, this protein, called green fluorescent protein (GFP), glows bright green.

The pair used green fluorescent protein (GFP) as the laser's "gain medium" , where light amplification takes place.

When labeled with a pBMP15-EGFP reporter, these cells further differentiate into round GFP-positive oocyte-like cells.

The green fluorescence protein (GFP) gene recombinant virus labeling is a new method for the neuroanatomical studies.

ObjectiveTo evaluate the efficiency for eukaryotic cell transfection by using GFP as a report gene in common transfection ways.

In animal models, a further enhancement in specificity can be obtained by GFP expression under control of tissue-specific promotors.

The genetic transformation efficiency could be deduced effectively by GFP reporter.

The fluorescent microscopy indicated that the D17-GFP was mainly localized in the luminal contents and mucus of the intestine.

To shed more light on the process, the research team used genetic engineering to make axolotls that produce GFP throughout their bodies.

When introduced to UV light, the GFP gobbles up photons, producing electrons that enter the circuit as electricity.

In 1992 Dr Prasher also cloned GFP, so that it no longer had to be harvested from living creatures.

Objective To establish a culture system for human embryonic stem cell and label the hES cells with GFP by lentivirus.

In the new work, cells derived from human kidney cells were genetically engineered to produce GFP.

Since GFP makes its own chromophore, it is perfect for genetic engineering.

Second, in isolated rat adipose cells, glucose transporter 4 trafficking can be visualized by transfection of HA-GLUT4-GFP.

Martin Chalfie demonstrated the value of GFP as a luminous genetic tag for various biological phenomena.

Transfection with full-length VP15 resulted in GFP fluorescence being distributed exclusively in the nucleus.

The detection results of stereo-fluorescence microscope show that green-fluorescent protein (GFP) gene is a kind of ideal marker gene.

PCR analyses showed that GFP DNA was not detectable in blood or distal tissues.

RESULTS The expression of GFP was obviously induced only in the curcumin group.

Objective To construct a recombinant AAV reporter virus expressing GFP and observe its expression in NIH 3T3 cell.

The remarkable brightly glowing green fluorescent protein, GFP, was first observed in the beautiful jellyfish, Aequorea victoria in 1962.

what are the advantages of tagging a protein with a fluorescent protein tag such as GFP?

Researchers worldwide now use GFP to track development of brain cells, the growth of tumors and the spread of cancer cells.

The story behind the discovery of GFP is one with the three Nobel Prize Laureates in the leading roles.

Amplified of the green fluent protein (GFP) gene from plasmid PCR3. 1, and add suitable restriction enzyme cutting site.

Results In the first and the second group the GFP in much muscle expressed, some fluorescence in the muscle was bright, some was dark.

Objective: To obtain GFP-RECK expression vector and to express it in glioma cell line SHG44.

Methods B16 melanoma cells were implanted subcutaneously in GFP mouse to copy GFP mouse tumor model.

Xenograft tumors were developed in athymic mice using MCA207 cells expressing green fluorescent protein (GFP).

Transient expression of the ZF3-GFP protein in onion epidermal cells showed that ZF3 was localized in cell nuclei.

Hence, because the gene product is easily detectable by its intense fluorescence, the gfp cDNA has become a unique reporter system.

The SNB: GFP fusion protein is localized to the nucleus.

Methods1. Cultivation of green fluorescent protein(GFP) neural stem cells and C6 glioma cells in vitro. 2.

Construction of a GFP-labeled Recombinant Adenovirus Harbouring GDNF and Its Expression in Rat Neural Cells in vitro and in vivo

Construction of the Transgenic Plasmid Containing GFP Gene and Four Ribozyme Genes Targeting the Prawn Baculovirus Gene

Construction and sequence analyzing of mammalian GFP- fused expression vector of osteoclast differentiation factor

Quantitative Analysis of GFP Expression Level With Fluorescent Spectrophotometer

Cloning of the gE gene of Pseudorabies virus Shanghai strains and construction of plasmid vector containing GFP gene

MRNA Functional Repair of a Mutant GFP Gene Using a Trans-Splicing Ribozyme

Preliminary Study of GFP Quality Control Microarray

Expression of GFP gene transferred into the fertilized eggs of Carassius auratus by microinjection

Integration and expression of GFP gene directed by the erythroid-specific element in transgenic mice

The Culture and Influence Factors on Proliferation of Skeletal Muscles Satellite Cells from GFP Transgenic Mouse in vitro

Establishment of cell line of mouse embryonic stem cells and its label with GFP

Construction of transgenic mice expressing GFP and Embryo Cryopreservation

Expression of Recombinant GFP-TNF Fusion Protein and Its Application

Expression of plasmid DNA encoding GFP mediated by cationic polymer after its intrastromal injection

Whole-body Fluorescent Imaging of the Growth and Metastasis of GFP-expressing Tumors

New Method for Spatial Structure Quantification of Biofilm with GFP Tagged Bacteria

Studies on Introduction of GFP Gene into Sheep Fetal Fibroblast Cell by Electroporation

Specific Suppression of GFP Gene Expression by Short Double Stranded RNA in Mammalian Cells

Study on A GFP Expression System for Detecting Estrogenic Compounds

Actin Visualization in Living Immature Pollen of Rice Using a GFP-Mouse Talin Fusion Protein

Construction of GFP and CMV MP Fusion Gene and its Expression in E. Coli

Improvement of AdCMV-GFP Gene Transfection Efficiency Induced by Heavy-ion Beam Irradiation on Murine Melanoma Cells

Photobleaching Properties of GFP Expressed in Human Lung Cancer Cells

Study of osteoblasts transfected with GFP in vitro and traced in vivo

Specific inhibition of GFP expression by small hairpin RNA in colon cancer LoVo cell

Retrovirus-mediated transfer of GFP gene into lens epithelial cells in vitro

Using GFP retrovirus to label tumor cells and vascular endothelia cells

Culture and identification of neural stem cells of GFP transgenic mouse

Molecular imaging research on the effect of endostatin on mice GFP-expressing Lewis lung carcinoma

Improvement of Genetic Transformation System by Using GFP as Reporter Gene on Pepper

Expression of GFP gene in brain mediated by immunoliposome injected into lateral ventricle

GFP and Its Application to Plant Research

The shh Promoter of Zebrafish Directs the Expression of GFP in Notochord

In vivo molecular imaging of GFP labeled Lewis lung cancer in mice model

The application of GFP in research of transgenic animals

Expression of GFP Report Gene under the Control of Bovine BLG Gene 5'Regulatory Sequence in Primary Mammary Epithelial Cells

GFP Does Not Sense All Fault Current. GFP

Study on GFP-labeling and mycoparasitism in Chaetomium globosum

The Expression of GFP Gene in Transformed and Tumor Cells

Use of GFP labelling technique to monitor tissue engineered bone formation in nude mice

Main Product and Market of GFP