plasmid

One of the ways to introduce genes into a bacterium is to introduce a plasmid into it.

Agrobacterium tumefaciens contains a plasmid, a circular piece of DNA separate from the bacterial chromosome, known as the Ti plasmid.

there was no bacterial plasmid backbone sequences inserted into MON88017 genome.

Agrobacterium tumefaciens is a soil bacterium with a plasmid that inserts foreign DNA into a plant.

The test demonstrates that if a second gene, insulin for example, were to be patched in the plasmid, that gene would be expressed as well.

He described the technique to put this gene inside plasmid cationic liposome complexes and then deliver it intravenously.

The new plasmid is inserted back into a cell, where numerous copies can be made.

Transfer of a segment of DNA to a new position on the same or another chromosome , plasmid, or cell .

The effective expression of this plasmid had been demonstrated by FACS and IMF.

The factors influencing survival rate of LAB and plasmid stability during lyophilization were systematically studied.

Plasmid is isolated from these cells and the yeast genomic fragments are isolated by digestion with an appropriate restriction enzyme.

The expression of the transferred recombinant human insulin gene retroviral vector plasmid implements safe regulation.

Methods: Experiment was divided into three groups: negative control group, blank plasmid group, interference fragment transfection group.

Objective To comprehend the resistant state of shigella and plasmid profiles of shigella isolated in Honghe area.

One plasmid isolated from bacteria in a sewage treatment plant was found to encode nine different antibiotic-resistance genes.

To transfer ( genetic material or characteristics ) from one bacterial cell to another . Used of a bacteriophage or plasmid .

Ion exchange HPLC is an alternative that yields milligram quantities of purified plasmid DNA within an hour.

Objective: To construct a recombinant plasmid carrying extracellular protease gene from Lactobacillus.

Objective To investigate calcium oscillations phenomenon, the eukaryotic expressing plasmid of human CNB was constructed.

Plasmid profile and ERIC PCR proved that the homology between infective strains and environmental isolates were existed.

Objective To analyze the plasmid and resistant spectra and the location of the resistance gene of Acinetobacter baumannii .

The Electroporation Experts Perform IM(intramuscular) injection of desired plasmid in the anterior tibialis muscle.

The relationship between plant mitochondrial plasmid DNAs and cytoplasmic male sterility (CMS) is discussed.

The recombinant plasmid with the hammerhead ribozyme gene was correct by digestion identification .

These novel vaccine strategies, including plasmid DNA, live recombinant vectors and combined vaccine.

The recombinant strain for glutathione synthetase systems is constructed by use of the glutathione synthetase-system recombinant plasmid.

Methods: Plasmid Conjugation experiments and Dot Blot hybridization were performed.

The ability of the cationic polysaccharide to incorporate and protect plasmid DNA was measured.

The invention also provides the preparative method and usage of the recombination plasmid.

Switch on the vacuum to draw the solution through the column (The plasmid DNA binds to the column material).

STUDY ON PLASMID FINGERPRINTING AND ANTIBIOTIC SUSCEPTIBILITIES OF SHIGELLA SPP.

It requires controlled plasmid delivery systems and tissue specific gene expression system.

Results: Escherichia coli strains with different drug resistance have the same plasmid profile.

Insert and ligate any DNA fragment into a vector such as plasmid or viral vector, to form a recombinant DNA.

Objective To survey the antimicrobial resistance and plasmid profiles of Neisseria gonorrhoeae isolated from a hospital.

Plasmid technology has also been developed for moving targeted genetic material into plants.

Transfer of genetic material or characteristics from one bacterial cell to another by a bacteriophage or plasmid.

The whole trials results showed injection of GRF gene plasmid could improve growth performance of calves.

The gene is carried on a plasmid, a small section of DNA that can move from one bug to another, passing on drug-resistance as it goes.

And the reconstructed plasmid was transfected into the keloid-derived fibroblasts with the help of lipofectamine.

Aim: To develop a PCR technique for rapid screening of recombinant plasmid in subtractive library of cDNA.

The first step in the process is to take our plasmid which we've selected, and to insert the gene that we want into it.

The positive rate of the R plasmid conjugation experiment was 51. 2% in norfloxacin-resistant strains.

Recombinant plasmid PDSBCM, microorganisms transformed therewith, and method for producing alkaline protease VAPK.

In this review, we summarize the current progresses on plasmid biology in the rare actinomycetes.

The plasmid was confirmed by restriction mapping and sequence analysis .

This review focuses on the present status of application of polymer-mediated plasmid DNA transit systems.

Objective To control the quality of mouse nerve growth factor (NGF) DNA plasmid for gene therapy.

The strains with recombinant plasmid of hEC-SOD were induced and expressed as inclusion body.

In this technique, scientists use the plasmid from a bacterium that causes tumors on plants.

Objective: To construct the recombinant adenovirus plasmid carring human PML gene for further study.

There is no evidence of the mutagenicity of the plasmid.

Objective To clone the human angiotensin-converting enzyme 2 (ACE2)and construct its eukaryotic expression plasmid.

Objective To develop a procedure for large-scale purification of plasmid DNA.

Methods: The plasmid was constructed by DNA recombination.

A bacteriophage , a plasmid, or another agent that transfers genetic material from one location to another .

Plasmid could be extracted from all the 8 strains, the further study was needed.

We constructed a recombinant plasmid of HSP70 with enhanced green fluorescent protein (EGFP).

The test explored eliminating effect of Melaphis chinensis Baker on R plasmid from P.

Amplified of the green fluent protein (GFP) gene from plasmid PCR3. 1, and add suitable restriction enzyme cutting site.

Recover the amplified fragment, inserted into expression plasmid pET28(b).

Study on transfering and diffusing of antibiotic resistant plasmid in Shigella spp .

In our previous work, we constructed a recombinant plasmid pbc-SBP and expressed recombinant SBP in E. coli.

Recobinant plasmid PSP1, microorganisms transformed therewith, and method for producing alkaline protease VAPK.

Through crosslinking the specific peptide and the gene of TSST-1, we got the recombination plasmid.

Molecular techniques, such as plasmid profiles, have also been of limited value in identifying individual strains of Vibrio cholerae.

In the end filter out the most interference efficient plasmid.

I focus on the role of antisense RNAs in plasmid replication.

The recombinant plasmid was transfected into the cell line HL-60 by electroporation.

Amplification VP7 gene from RV G4 serotype, construct a plasmid includes this gene and clone culture.

Objective: To find a rapid method of screening positive recombinant plasmid.

Finally a recombinant plasmid named pcNcSRS2, which was identified by PCR, restriction enzyme analysis and sequencing, was obtained.

Curing of the plasmid results in a loss of the ability to synthesize the parasporal body, a discovery made in 1977.

Objective To study the relationship between the resistance R plasmid and both pili and adherence of Pseudomonas aeruginosa strain, PA36.

Isolation and identification of a Bifidobacterium strain with a plasmid polymerase by PCR

Experimental study of total nutrient admixture promoting transfection of plasmid DNA mediated with liposomes to colorectal cancer cells

Construction of Prokaryotic Expression Plasmid of the Human Tumor Suppressor Gene PTEN and Its Expression in E. Coli

Study of the expression of exogenous augmenter of liver regeneration recombinant plasmid in liver tissue of rat

Construction of an eukaryotic expression plasmid for short peptides used in DNA immunization

Construction and expression of a eukaryotic plasmid containing luciferase gene regulated by partial

Co-expression plasmid construction of vascular endothelial growth factor and Fas ligand and detection of its expression in cultivated cells

The construction of a chromosome-integrated plasmid for the replacement of diphtherial toxin gene

Application in Controlling Environmental Persistent Organic Pollution Compound of the Natural Degradation Plasmid

Construction of a low molecular weight plasmid vector for the capture of genes encoding exported proteins

Construction and expression of a secretory-fusion plasmid containing human angiostatin gene

Construction of recombinant plasmid carrier including the principal immunocompetent domains of rabies virus glycoprotein and nucleoprotein

Construction of eukaryotic recombinant plasmid of herpes simplex virus thymidine kinase gene and its expression in skin tissue of scald rats

Construction and Expression of an Eukaryotic Recombinant Plasmid from a Hybrid Gene of Antigen for Plasmodium falciparum

Construction and Expression of a Eukaryotic Plasmid Induced by Bone Morphogenetic Protein

Construction of Expression Plasmid Encoding Fibroin Crystalline Domain Like Protein of Silkworm and its Expression in E. Coli

Cloning of the Human Angiotensin-converting Enzyme 2 Gene and Its Eukaryotic Expression Plasmid Construction

Construction and identification of a reporter plasmid for analyses of Notch signaling pathway

Study on Plasmid Stability of a Recombinant Escherichia coli Producing Nitrile Hydratase

Experimental study of neurogliocytoma apoptosis induced by TRAIL eukaryotic expression plasmid

Construction of a plasmid for expression of foreign epitopes as fusion protein with subunit B of cholera toxin

Gene Sequencing of Campylobacter jejuniCommon Antigen Recombinant Plasmid and Property Analysis of Its Expression Products

The Construction of Recombinant Plasmid with Prostate-specific Membrane Antigen Promoter Controlling Reporter Gene Expression

Identification of recombinant expression plasmid by restriction endonuclease digestion

Construction of the Recombinant Expression Plasmid for the Expression of the Whole Anti-D Immunoglobulin Molecules

Construction and identification of recombinant shuttle adenovirus plasmid containing glialcellline derived neurotrophic factor

Development of a new suicide plasmid and its application to Brucella mutant construction

Experiment treatment of collagen-induced arthritis in rats with recombinant plasmid containing vasoactive intestinal peptide gene

Construction of Eukaryotic Expression Plasmid Encoding Human Carcinoembryonic Antigen

Antibiotic Resistance and Plasmid Profile of Thermotolerant Coliform Bacteria Isolated from Aquatic Environment

Construction and Expression of Mouse Endostatin Eukaryotic Plasmid and Its Bioactivity in vitro

Construction and Identification of Eukaryotic Expression Plasmid of Resistance-related Chymotrypsin Gene

Construction of the shuttle plasmid vector for fusion protein gene and expression in Hepatocellular Carcinoma cells

Construction of the Transgenic Plasmid Containing GFP Gene and Four Ribozyme Genes Targeting the Prawn Baculovirus Gene

Construction of Recombinant Plasmid for Fusion Expression of Human Cytomegalovirus and Identification of Expressed Product

Inhibition of corneal neovascularization by liposomes mediated plasmid encoding human endostatin

Construction of Mouse Endostatin Expression Plasmid Recombinant and Its Expression in E. Coli

Study on Antibiotic Resistance and Plasmid Profiles of Two High Efficient Strains of Rhizobium sp. Acacia

Expressions of Liposome-mediated and Naked Plasmid DNA Encoding Human Atrial Natriuretic Peptide in Cultured Rat Ventricular Myocytes

Gene therapy for diabetes mellitus: construction and identification of eukaryotic cell expression plasmid in rat proinsulin gene

Study of clone and reconstruct prokaryocyte expression plasmid of BLCAP gene

The construction and identification of the plasmid expressing green fluorescent protein

DNA Reconstruction of plasmid DNA with Red-mediated homologous recombination combined with restriction digestion

biosynthesis genes; Suicide plasmid; Gene targeting; RNA interference; Apoptosis;

Study on the Resistance to Non-Specific Immunity of the Host Bacteria Mediated by the Drug Resistant Plasmid

Study of plasmid curing methods and appraisal of plasmid elimination effect

Bioactivity and safety of direct intramyocardial injection of naked plasmid DNA encoding human atrial natriuretic peptide

Plasmid DNA: Investigation of Integration into Host Cellular DNA Following Absorption Via the Gastrointestinal Tract

Mitochondrion like-plasmid identification of a new male sterile cytoplasm WB in Zea mays

Construction of eukaryotic expression plasmid encoding Fba protein of group A streptococcus and elicitation of immune responses by it

Construction of Recombinant Plasmid for Co-expression of Nucleoprotein and Glycoprotein of Rabies Virus aG Strain in Eukaryotic Cells

Competition between Plasmid-bearing and Plasmid-free Organisms in the Host: Population Dynamics and Antibiotic Resistance

Effect of Complex Coordinating Tea Polysaccharide with Cerium on Degradation of Plasmid DNA and Organophosphorous Pesticides

Construction of Eukaryotic Expressing Plasmid of Mouse TRAIL and Its Inhibitory Effect on Tumor Growth

Construction of RNA interference expression plasmid of human neuropathy target esterase by cloning reverse repeats into plasmid DNA

Transient expression of eukaryotic expression plasmid CEA in vitro and its detection

Construction of a fusional expression plasmid in human monocyte chemoattractant protein-1

Surveillance of the antibiotic resistance and plasmid of Salmonella paratyphoid

Cloning of the gE gene of Pseudorabies virus Shanghai strains and construction of plasmid vector containing GFP gene

Construction of eucaryotic expression plasmid carrying the recombined human BMP-7 gene and its expression in chondrocytes

Construction of sequencing recombinant plasmid and eukaryotic expression recombinant plasmid of ESAT-6 mycobacterium tuberculosis

Plasmid Transfection of Rat Bone Marrow Mesenchymal Stem Cells by Cationic Lipid for Gene-modified Cell Transplantation Therapy

DNA Large-scale Purification of Plasmid DNA by Ultrafiltration with Hollow Fiber Filter and Hydrophobic Chromatography

Cationic polymer mediated plasmid encoding EGFP retrograde transfection in retinal ganglion cells

Construction and Sequencing of the Plasmid Carrying Three Copies of Glucose Responsive Element

Construction and Expression in E. Coli of Recombinant Plasmid for the Monoclonal Antibody against Platelet

Cloning of Human Phenylalanine Hydroxylase Gene and Construction of Prokaryotic Expression Plasmid

Bovine Enterokinase Catalytic Subunit cDNA Cloning and Construction of Fusion Expression Plasmid

Characterization of gene expression following immunization with nucleic acid plasmid

Construction of an Eukaryotic Expression Plasmid for gE Gene of Pseudorabies Virus Strain Yue-A

Shuttle plasmid construction of Signature-Tagged Mutagenesis technology

Molecule epidemiological study on a plasmid profile obtained from Guangxi Yersinia pestis

Construction and Identification of Shuttle Plasmid of Recombinant Adenoviral Vector Carrying Rat GDNF Gene

Effective labeling of stem cell transplantation in vivo: the application value of green fluorescent protein plasmid labeling

Cloning of human TGFBI gene and its eukaryotic expression plasmid construction

A Simple and Rapid Method for Extraction of Plasmid DNA and its Detection

Construction of Human Polypeptide: N-Acetylgalactosaminyltransferase 2 Gene Antisense RNA Expression Plasmid and Its Functions

Effect of IFIT3 gene in the pathogenesis of systemic lupus erythematosus and construction of its eukaryotic expression plasmid

Construction of the recombinant prokaryotic expression plasmid contain BAG domain in Arabidopsis thaliana

Injection of naked plasmid using hydrodynamics-based procedure: an excellent approach in gene therapy research

Antibiotic Susceptibility and Plasmid DNA Isolation for Bifidobacteria

Construction of recombinant plasmid of Pseudomonas aeruginosa exotoxin A and its secreting expression in E. Coli

Construction of murine IL-12 eukaryotic expression plasmid of single chain fusion gene and its potential in gene therapy of cancer

Construction and sequencing of plasmid containing human secretive endostatin

Therapeutic effects of augmenter of liver regeneration recombinant plasmid on rats with hepatic fibrosis

Construction of recombinant eukaryotic expressing plasmid of mouse IL-18 and analysis of its gene polymorphism

Construction of FL gene eukaryotic expression vector and the hFL expression of this plasmid

The effect of a mouse IL-12 plasmid on airway inflammation and cytokine production in a mouse asthmatic model

Construction of Recombinant Plasmid and Standard Curve for Detection of PrP Gene Expression by Real-time Quantitative PCR

Construction of murine interleukin-12 eukaryotic expression plasmid and its expression

Effect of octreotide on bacterial translocation in severe acute pancreatitis: a study by recombinant plasmid labeling

The comparative efficacy of recombinant adenovirus and plasmid in treatment of myocardial ischemia

Construction of Plasmid with Green Fluorescent Protein Gene for Analysis of Yeasts in Yeast Wastewater Treatment

Construction of the lentiviral expression plasmid carrying OPCML gene

Plasmid Conjugation Method for High Level Gentamicin Resistant Enterococcus

Surveillance on antibiotic resistance and plasmid of salmonella paratyphoid

Isolation of Lactic Bacteria against Pathogen, Drug Susceptibility and Extraction of Plasmid

A Comparison of the Extraction Methods of Plasmid from Acrylamide Degrading Bacteria

Construction and identification of eukaryotic expression plasmid of human growth hormone

A study on purifying of vascular endothelia growth factor plasmid by a new type of magnetic nanoparticles

Detection of Plasmid from Tetrahydrofuran Degrading Bacteria and Study on Their Growth Properties

A Novel Method for Assaying Enzymatic Activity of Ribosome-inactivating Protein Using Plasmid DNA as Substrate

Plasmid DNA Distribution and Gene Expression Generated via Tail Vein Hydrodynamic Injection

Construction of eukaryotic expression plasmid for human basic fibroblast growth factor gene

Using bioinformatics to identify the unknown character of HIV sequence in a plasmid

Construction and sequences analysis for eukaryotic expression plasmid of foot-and-mouth disease virus

Removing drug tolerance and drug-resistance plasmid of Escherichia coli using Chinese herbal medicine

Effects of naked plasmid expressing human erythropoietin for the treatment of renal anemia

Construction of Secreting Plasmid for TRAIL and Its Inhibitory Effect on Liver Cancer

Construction of eukaryotic expression plasmid of insulin-like growth factor 1 and its expression in rat spinal cord

The Construction and Expression of Eukaryotic Expression Plasmid of Legionella Pneumophila Immunogenic Protein Gene

Construction of recombinant plasmid pEGFP- NGB and its expression in cultured neuroglial cells

Polymerase chain reaction of the recombinant eukaryotic expression plasmid of humaninsulin gene

Construction and Identification of Eukaryotic Expression Recombinant Plasmid Containing Mice Connexin 43 Gene

Measurement of ofloxacin-resistant plasmid of pathogenic Escherichia coli from chicks

Expression of plasmid DNA encoding GFP mediated by cationic polymer after its intrastromal injection

Stability and copy number in host bacteria of a new plasmid cloning vector

The construction of eukaryotic expression plasmid for human thrombomodulin gene

Construction and Expression of Recombinant Wild Lipoprotein Lipase Gene Plasmid

Construction of the prokaryotic and eukaryotic recombinant plasmid of mip gene of legionella peneumophila

Construction and expression of recombination plasmid containing mouse secretable endostatin gene

Construction and expression of eukaryotic expression recombinant plasmid of ESAT-6 from Mycobacterium tuberculosis

Immune Responses in Mice Induced by Plasmid DNA Expressing gD of Infectious Bovine Rhinotracheitis Virus

Construction of secretory human interleukin 18 plasmid and its expression in eukaryotic cells

Assistant Units of Plasmid DNA and Its Application in Gene Immunization

Construction and Sequence Determination of Recombinant Plasmid of PTEN Gene Fragment

Construction and confirmation of the fused plasmid with Rb bait gene in yeast two - hybrid system

Naked plasmid mediated expression and therapeutical effect of mutant human proinsulin gene in diabetic mice

Construction of Brucella Unmarked Deletion Mutant by Using Conventional Cloning Vector as Suicide Plasmid

Plasmid with Resistance to Neisseria Gonorrhoeae-Sensitive Antibiotics: an Analysis in Zhongshan City of Guangdong Province

The Study of the Plasmid and Restriction Endonucleases Map of Y. Pestis in the Northwest Pastures in Jilin Province

Construction and expression of A eukaryotic plasmid encoding murine VCAM-1 in vitro

A Rapid Extraction of Plasmid DNA and Its Application in Mass Screening of Recons

Effects of injecting plasmid DNA into toad skeletal muscle on its contractility

Construction and Identification of Recombinant Shuttle-plasmid with ESAT-6 from Mycobacterium tuberculosis

Cloning of Influenza A Nucleoprotein and Constructing the Recombinant Plasmid

Construction of Recombinant gAd Plasmid and Its Expression in E. Coli

Influential factors on the efficiency in transfecting human keratinocytes with plasmid-liposome complexes

Effects of the two drugs on the antibiotic resistant plasmid of Enteric bacilli

Research of construction, transfection and expression of human bone morphogenetic proteins factor-2 eukaryotic plasmid

Influence of Invasive Plasmid Antigen on the Immune Protection of Bivalent Shigella Vaccine

Improving experiment on the method of rapid identifying positive recombinant plasmid

Construction and Identification of Small Interfering RNA Plasmid Specific for BACE in vitro

Experimental study on construction of recombinant t-PA gene plasmid to prevent the thrombosis after mechanical valve replacement

Expression of Foot-and Mouth Disease Virus Proteins in vitro with Eukaryotic Expression Plasmid

The evaluation of protective efficacy of tuberculosis DNA vaccines with different dosage and by codelivery of cytokine plasmid

construction of expression plasmid vector containing MARs;

Mechanism of plasmid-mediated quinolone resistance of Klebsiella

Construction of eukaryotic expression plasmid of human platelet-derived growth factor-B

Optimization of expression of double genes co-expression vector and plasmid stability

Construction and Identification of Eukaryotic Fluorescent Expression Plasmid of Glucagon-Like Peptide-1 Gene

Bacterial Plasmid Extraction Methods for Several Bacteria

Analysis of Staphylococcus Resistance and Plasmid Profile

Study on dose-chemotactic and antitumor effects of SLC eukaryotic expression plasmid

Construction and Expression of Eucaryotic Recombinant Plasmid of Legionella pneumophila mip Gene

Influence of Plasmid Preparation and Temperature on Nested Deletions

Metabolic kinetics of foreign plasmid DNA uptake via gastrointestinal tract in mice

Subcutaneous injection of plasmid VEGF gene: a method of gene therapy to enhance the viability of random skin flap

Modified PCR Methods for Identification of Recombinant Plasmid

Control Mechanism of Bacterial Plasmid Replication

Construction of alpha-2 macroglobulin cDNA-enhance green fluorescent protein expression plasmid

Construction and Identification of the Prokaryotic Expression Plasmid of Human MMP-1 Gene Fragment

Effectual analysis of preserved folliculostatin gene recombination plasmid transfected E. Coli by cold-preservation versus freezing

Key Influential Factors and Selection Methods of Plasmid Extraction

An analysis of resistance plasmid and resistance of Pseudomonas aeruginosa infection in respiratory tract

Construction and verification of the dual-promoter plasmid pCN-SSISG as bivalent anti-caries DNA vaccine

Construction of cow reproductive system PrP gene standard plasmid and curve using real-time RT-PCR

DNA Rapid Preparation of Plasmid DNA for Overnight Restriction Digestion

Construction of human MAGE-3 eukaryotic expression plasmid and its expression in vitro

Construction of human TIMP-2 eukaryotic expression plasmid and its expression in vitro

A Improving Method for the Plasmid DNA Isolation from Cyanobacteria

Construction of X-gene defect HBV expression plasmid and cells transfection study

The three target genes of the standard plasmid were cloned respectively to establish a quantitative assay;

Effects of Temperature Induction Modes on the Plasmid Copy Number in Escherichia coli

The Expression of Dendritic Cell Transfected with Plasmid Carcinoembryonic Antigen

Construction of human motilin receptor gene expression plasmid