PCR

Another result of the widespread adoption of PCR was that radioisotopes would no longer be used in DNA-based forensic investigation.

As for PCR testing of biological samples from abductee experiences he indicated, You might imagine that I thought of that myself.

PCR analysis of the total DNA extracted from transgenic plants has shown that the target gene had been integrated into the rice genome.

TED mentions that Mullis invented PCR and surfs and seems to skim over a few other things.

It shows that this DNAarray can be applied to monitor the swine influenza, the sensitivity is higher than PCR.

Ten-fold dilution method was used to detect the sensitivity and compared with the general PCR method and culture method.

Furthermore, sugarcane ratoon stunning disease pathogen can be rapidly detected by means of television phase contrast microscope and PCR.

PCR technique is usually applied in the detection and identification of specific bacteria in the fermentation.

Objective: To set up a real-time fluorescent quantitative PCR assay for rapid detection of norovirus(NV) RNA in acute gastroenteritis.

The PCR products of suspected cases with the point mutation were further DNA sequenced so as to determine its mutation characters.

It has the key features of higher reproducibility, low false positives, speed and cost-effectiveness and a wide range of PCR products.

These results indicated that the multiplex PCR-DHPLC assay can be used for the specific and sensitive detection of Campylobacter jejuni.

Results The aim gene fragment can be amplified by a multiplex PCR assay, it largely reduce the time of identification.

The companies that sell professional-grade PCR machines to laboratories are predictably unimpressed by all this fuss.

The electrophoresis result of PCR production was satisfactory with clear strap, correct place and non-specificity amplification.

Two of the PCR products were sequenced . One was an unknown gene and the other was similar in sequence to the actin gene of pea.

Meneley, however, contests claims that the PCR is simple to calculate, especially in a small reactor like the Maple design.

PCR stands for Polymerase Chain Reaction and is a common way of testing for a variety of different organisms.

After a predetermined time, the total RNA was isolated from cells and the differential mRNA expressions were evaluated by RT-PCR method.

There was no difference in neisseria gonorrhoeae culture, glucolysis test and PCR test.

If the number of neutrons increases, then the PCR is positive; if it decreases, PCR is negative.

Polymerase chain reaction is really just a process of heating and cooling genetic material.

The sequencing results on the amplified products of positive PCR showed that CBF1 gene from rape had been transformed into A. thaliana.

She points out that with the Maples, it was not the positive PCR itself that was the problem.

Responsible for PCR member recognition program and any other loyalty based initiatives.

Polymerase chain reaction (PCR): Laboratory technique used to make numerous copies of specific DNA segments quickly and accurately.

To give researchers more genetic material to work with, a PCR instrument repeatedly heats and cools an original biological sample.

PCR using human DNA obtained whole blood can be applied to identify genetic diseases and polymorphisms, or detect microbial infections.

PCR is not a suitable confirmation method to detect the infection of syphilis among the exit-entry people.

The result of PCR test shows that these clones have the same conserved positions as the cloned resistence gene.

Real-time PCR, as one of the rapid and sensitive methods, has been widely used to detect and identify Campylobacter jejuni.

In Mexico itself, a lack of RT-PCR equipment could be behind the confusion over the number of cases and deaths caused by the virus.

I -- you know, when I -- after I had invented PCR, I kind of needed a change.

The sensitivities of the samples were compared between the culture method, general-PCR and fluorescence quantitative PCR assay.

Results The DNA concentration and yields from different methods were similar, without influence on the result of RAPD-PCR.

The bottle can be manufactured in a variety of material types, including clear and coloured PET and 50% post-consumer recycled PCR polymer.

Unfortunately, many human skeletal remains do not contain original DNA amplifiable by polymerase chain reaction (PCR).

When the system boots, it measures its own hashing and PCR loading code and writes the hash to the first PCR of the TPM.

Compared to the 'reference' standard (histopathology), the PCR method had a sensitivity of 83% and a specificity of 66%.

PCR is used to measure the patient's molecular response (the extent to which the BCR- ABL gene is decreased).

PCR is a method for amplifying a small amount of DNA or RNA into large quantities in a few hours.

The reason for this difference might be related to the different automated sequencer methodology and the PCR slippage.

In the research, we introduce Listeria monocytogenes detecting methods by means of serology, PCR, monoclonal antibody and apparatus.

QPRIMER creates an electronic genomic PCR image from a set of conserved primers as well as summary pages for primer alignments and products.

Suppression subtractive hybridization (SSH) is a method produced by the combination of suppression PCR and subtractive hybridization.

Methods Polymerase chain reaction (PCR) was used to check and measure the tubercle bacillus DNA(TB-DNA) in malignancy tissue.

Two assays based upon PCR detection of a polymorphic PDR1 retrotransposon insertion in Pisum sativum have been developed.

Scientists commonly use a method called polymerase chain reaction (PCR) to create copies of a specific part of DNA.

Method The canine multiplex system were used for PCR reaction and the product were analyzed by ABI310 genetic analyzer.

ERIC-PCR can be applied in source-tracing, providing effectively technical support for prevention and control of food-poisoning events.

Conclusion The PCR-SSP may be used for genotyping in mutational mononucleotide, and it has a good prospects.

ACR varies between the MCR and the PCR, and is dynamically controlled using congestion control mechanisms.

Biochip technology is a great breakthrough of life science after recombinant DNA technology and polymerase chain reaction.

The growth curve and the result of Real-time PCR showed that 3% was the optimum concentration of pyrrhotite for YN-3.

The first method uses a dot assay for PCR product detection which could be fully automated for handling thousands of samples.

Methods: Polymerase chain reaction(PCR), DNA direct nucleotide sequencing and RFLP were used in mutation analysis.

Conclusions: Using PCR amplification products to prepare gene chip probe was a quickly, simple effective method.

But many in the nuclear industry vehemently disagree, and a number argue that the positive PCR is not in itself a big deal.

Using reverse transcription PCR, kininogen mRNA was also detected in lamprey gut, kidney, and leukocyte, but absent in lamprey buccal gland.

Single primer PCR was a method of chromosome walking to isolate sequences flanking a known DNA sequence with only one primer.

The technology of RT-PCR proves to be a rapid, accurate and reliable way to do genetic subtyping of influenza A viruses.

Only one pathogen can be detected in single PCR system which results in miscellaneous cost and trivial operations.

The aim of this review is to introduce and summarize the establishment, development and application of this method in Campylobacter jejuni.

The corresponding genes of those differently expressed fragments were validated by Real -time quantitative PCR.

Plate culture method combined with PCR-DGGE was applied to monitor the bactericidal efficacy of acidic electrolyzed water on shrimp.

Reverse transcription polymerase chain reaction (RT-PCR) detection was used to verify the differentially expressed 3 genes.

The results of GUS histochemical assays and PCR amplification demonstrated that the overall transformation rate of Roselle callus was 4%.

Conclusion PCR method is better than isoenzyme analysis in differentiating An. minimus A and C.

Coordinator of the PCR, a department responsible for the factory outside of the tracking problem rectification.

Additionally, the inhibitors would also interference with the PCR amplification to generate false-negative detection results.

In this paper, the basic principle of real-time PCR and its application in the detection of Mycoplasma hyopneumoniae were reviewed.

the amelogenin detection is most suggestible for the human tissues from which, enough quantity of genomic DNA is available.

RT- PCR assays were used to determine expression of CVB_3 - associated genes in cardiomyocytes.

Conclusion PCR with the general primers is suitable for rapid detection of fungal corneal ulcer because of quickness and high positive rate.

Electrophoresis of the PCR products was identified, and finally PCR product was purified by gel extraction kit to prepare for digestion.

However, it was recently reported that EU genotype PRRSV was positive by RT-PCR from submitted clinical samples suggestive of PRRS.

Results: Sequences were aligned , and the results showed that the products of PCR amplification were the specific gene fragments of HDV.

As the third PCR technology, Miniaturized flow-through PCR chip can amplify DNA specifically in an exponential fashion in vitro.

Specific primer PCR was a gene cloning technique with high efficiency, stability and accuracy.

meanwhile, methicillin resistant staphylococcus aureus(MRSA) were tested by cefoxitin method, oxacillin method and PCR.

Objective To discuss the feasibility and superiority of diagnosis of acanthamoeba Keratitis using polymerase chain reaction.

Plasmid profile and ERIC PCR proved that the homology between infective strains and environmental isolates were existed.

On the whole, the prediction precision of PLSR model was better than PCR's for both sand content and moisture content predictions.

PCR-embedded system for data storage characteristics, the scheme of using USB equipment as the interface is put forward.

Methods Polymerase chain reaction was used to detect Spotted fever Group Ricketfsiase DNA in tick and rodents spleen.

Its potentials and future perspectives in food fermentation and corresponding natural conditions are reviewed in this paper. . .

Aim To make up a stable method of in situ PCR and to probe the relationship between HPV infection and larynx squamous cell carcinomas.

RT-PCR result confirmed the reverse transcription of this gene in the genetically modified bacteria.

To investigate the application of the polymerase chain reaction (PCR) in diagnosis of oral candida leukoplakia.

Conclusions This result indicate that PCR primer introduced V region N terminal changes may seriously affect the antigen binding activity.

Result: Compared with normal PCR, Gradient PCR could identify the the optimal annealing temperature of aldosterone synthase gene.

Now PCR were used to detect these five major immunosuppressive virus and the results were good.

All isolates were typed by mycobacterial interspersed repetitive units (MIRU) and deletion-targeted multiplex PCR (DTM-PCR).

PCR can be used as an effective tool to investigate the distribution pattern in experimental MRL.

Nested PCR requires two sets of primers which are used to amplify a specific DNA fragment using two separate runs of PCR .

According to AECL, uncertainty about the cost of solving the PCR problem was a key factor in the project being scrapped.

Allele-specific diagnostic PCR authentication of Dendrobium huoshanense and its allied species of Dendrobium Sw.

The indirect in situ PCR technique may provide a feasible new tool pathological detection of Toxoplasma DNA using normal paraffin sections.

Methods PCR and asymmetric PCR were employed. The amplified fragments were separated on denaturing PAGE and visualized by silver stain.

Multiplex PCR and real-time PCR are developed for simultaneous and quantitative detection of bacteria-producing biogenic amine.

Detection of Candida Albicans in Blood by PCR in A Rabbit Animal Model with Disseminated Candidiasis .

Each lab now has a real-time polymerase chain reaction (PCR) machine, and one lab has two machines.

Canine brain samples from endemic areas were also collected for antigen detection using immunofluorescence assay (IFA) and RT-PCR.

The genotype-specific primers PCR method is a simple, rapid typing one that has good application value.

It was validated that the extracted DNA could meet the need of polymerase chain reaction (PCR).

Polymerase chain reaction-single strand conformation polymorphism, PCR- SSCP .

The technical principles of PCR-DCGE and its application in food industry (microbial ecology) were introduced briefly in this paper.

Conclusion PCR detection of pleural biopsy specimen had greater diagnostic value in tuberculous pleuritis .

Recently, an innovative dry-reagent-based PCR has been developed that may be used in district hospital laboratories.

Multiplex PCR technology can resolve this problem efficiently, thus making the detection of pathogens more simple and prompt.

Moreover, an involvement of the GGT promoter II was detected after RT-PCR and transient transfection studies.

These optimized multiplex-PCR-sets provide a technical basis for genetic diversity study and parentage test of domestic buffalo.

Methods After amplifying the virus by cell culture, influenza virus was differentiated by using RT-PCR and hemagglutination inhibition test.

It produces optimal and homogenous primer pairs that meet the need in experimental design with large-scaled PCR amplifications.

Objective To establish a method for rapid detection of clinical suspect fungal corneal ulcer by polymerase chain reaction(PCR).

The rRT-PCR Swine Flu Panel diagnostic test was authorized for testing samples from flu patients to determine if they have the new strain.

In this paper, the progress on researches and applications of PCR in the detection of food borne pathogenic microorganism were reviewed.

Polymerase chain reaction(PCR) extensively utilized for research about oral bacteria recently.

The PCR assay is specific and sensitive and can be used to verify the presence of BV.

Objective To detect Plasmodium falciparum with the Fluorescent Quantitative PCR(FQ PCR) and value this method.

Objective To evaluate the results of real-time PCR in detecting enteric pathogens in practitioners in the fields of food and public service.

Identification of transgenic plants by PCR revealed that the constructs had integrated in the plant genome.

In conclusion, ISSR-PCR is sensitive and stable for the molecular identification of Trichinella genus.

Hydraulic gun. PCR instrument. Meteorological chromatography. Freeze-drying machine, and other products.

To establish a TaqMan-based real-time fluorescent quantitative PCR assay for detection and quantitation of Hepatitis E virus.

Objective: To apply PCR-Pyrosequencing technology on samples of food poisoning for rapid detection of Salmonella.

Methods: designing cynomolgus monkey MHC-I B allele specific primers, amplified by PCR to obtain full-length alleles.

Objective: To determine the clinical value bacteria for detection of MTB FQ-PCR in sputum and blood.

Furthermore, the reaction system and program of ERIC-PCR method for the acid mineral drainage were optimized.

Aim To establish a PCR-MPH method for telomerase activity and its clinical application.

For his invention of the polymerase chain reaction (PCR) method.

DNA was isolated from peripheral blood samples from the subjects, amplified by PCR and sequenced, then compared with NCBI gene bank.

Objective To identify the species of cultured cells and to detect inter-species cross contamination by polymerase chain reaction (PCR).

Objective To establish DNA typing for HLA-DR antigens by polymerase chain reaction with sequence-specific primers (PCR-SSP).

Here, a detailed protocol for bisulphite conversion, primer design, and optimization of PCR conditions is given.

Objective: To research the indirect in situ PCR technology on the diagnosis of toxoplasmosis lymphadenitis.

Methods: Human leukocyte antigen B27 genome conservative region upstream primer design and downstream primers, optimizing PCR system.

A method of amplifying DNA, PCR multiplies a single, microscopic strand of the genetic material billions of times within hours.

A novel combinatorial multi-site directed mutagenesis strategy was developed, which performed in three stage PCR in one tube.

In addition PCR technique was used to detect the presence of the integron I and the multi-resistance genes.

Studies on the Establishment and Preliminary Application of Double PCR Technique for Detection of Malaria.

Establishing the technique by using reverse transcription polymerase chain reaction(RT-PCR) to detect one virus or several viruses.

The relative copy number of the shuttle vector pZL1 in Gluconobacter oxydans was determined by real-time quantitative fluorescent PCR.

Companies must make an initial investment in a PCR instrument and train personnel to use it.

The double strand probe was hybridized with DNA of liver, bone marrow, and spleen, stomach, small intestine, colon. and sera.

Polymerase chain reaction (PCR) is perspective for detection of animal derived feedstuffs .

Development and application of multiplex PCR assay for the detection of Campylobacter spp.

Through comparing the different reaction conditions, the ISSR-PCR reaction system was optimized.

The results of RT-PCR and RT-PCR-Southern blot show that the target gene had been transcripted.

The viral RNA was amplified by a reverse transcription polymerase chain reaction (RT-PCR).

Real time quantitative PCR has been widely used in various areas of plant pathology research.

The QR-PCR was modified to investigate whether virus exists or even replicates in peripheral blood mononuclear cells.

Both genes were correctly cloned and identified by PCR, restriction enzyme digestion and sequencing.

The positive samples obtained by fluorescence PCR were higher than those ordinary PCR and culture method in sample test.

and a detection method for PCR amplified products.

The optimum annealing temperature of different primers was decided by gradient temperature PCR.

Aim To establish multiple PCR method for rapid detection of Candida albicans and non-Candida albicans medical fungi in one test.

Methods HLA and KIR genotypes were detected by sequence specific primer-polymerase chain reaction (PCR-SSP).

The best condition for PCR response was studied, and the PCR reaction had high ability and specificity.

The results of PCR demonstrated the integration of target gene into oil sunflower chromosome primarily.

Then the subtracted library was screened by PCR and the differential fragments were sequenced and analyzed with BLAST.

Conclusion AP-PCR may be useful in the identification and classification of SSG species.

Panhandle PCR is a quick and convenient new technique for cloning and identifying unknown partner genes.

The results of PCR test for samples consistent with the results of clinical and pathological diagnosis.

Methods: Shellfish samples of Dalian area were detected by fluorescent quantitation PCR and ELISA.

All specimens handled were tested for detecting M. tuberculosis by PCR-reversed dot hybridization.

Objective To observe the rapid diagnosis value of multiplex PCR in patients with community-acquired pneumonia.

Objective To develop a RT-PCR method for detecting gastric cancer micrometastases in axillary lymph nodes.

Finally, repetitive-element PCR was used to analyze subtype of C. perfringens.

Multiplex PCR which can simultaneously detect more than one bacteria has been applied increasingly and widely.

It was put forward that RT-PCR is a fast and accurate method to diagnose atypical hog cholera.

abstract: Objective To explore the clinical value of ELISA combined with PCR determination in patients with pneumoniae mycoplasma infection.

The PCR technology is used in laboratory animal pathogenic dermatophytes detection, it is a simple and time saving method.

The PCR products were cloned and sequenced. The sequence was compared with other ehrlichiae for homology.

The research and application situations of PCR-DGGE technology in aquaculture were reviewed.

The kinematical equation of a 3-PCR parallel robot was analyzed in geometrical method.

Method: PCR amplification, DNA sequencing and cladistic analysis.

PCR and sequence analysis were used to detect the toxin gene.

Gene names, accession numbers, sequences, PCR primers, and file upload are accessible for input.

The PCR products were analyzed by electrophoresis in agarose gels stained with ethidium bromide.

An agarose for molecular screening that improves resolution of small DNA fragments and PCR products.

Methods YAP element was detected by PCR amplification and agarose gel electrophoresis.

G1 gene sequence of M fragment from hantavirus genome was amplified by reverse transcription polymerase chain reaction(RT-PCR) and analyzed.

Objective To develop a nested-polymerase chain reaction (PCR) to identify G and P genotypes of group A rotavirus.

and to evaluate the value of nested polymerase chain reaction(nested-PCR) in distinguishing tuberculosis and sarcoidosis.

In this paper, the basic principles, main types and detection applications were introduced.

Aim: To develop a PCR technique for rapid screening of recombinant plasmid in subtractive library of cDNA.

The RT-PCR was adopted to detect the expressions of resistin gene in epiploon and subcutaneous fatty tissues.

The PCR product was subjected to electrophoresis and the results were analyzed by gel imaging and analysis system.

The elongation gene expression of developing cotton fiber cultured in field and in vitro was detected by using relative quantitative RT-PCR.

Conclusion A multiplex PCR method has been developed for the detection of A. cantonensis larvae in P. canaliculata.

In addition, the PCR united oligonucleotide probe improve the specificity and sensitiveness of the measured.

As a screening tool, Slezak sees the LLMDA as occupying niche roles between PCR machines and sequencing.

This study provided a method for PCR detection fish-derived material in ruminant feedstuffs .

In this study, we develop a new screening method by extracting and purifying microbial DNA from soil directly and PCR.

The PCR products were further analyzed by sequencing and nucleotide alignments.

Then cell is cracked by cracking liquid with SDS, the liquid also has PVP that with absorption to PCR restrictor.

Similarly, entire countries that lack RT-PCR capacity will send their samples to other countries for analysis using the WHO courier system.

Using this technology, a novel method has been developed for quantitative assay of telomerase product by real -time PCR.

Conclusion Nested PCR is a specific and sensitive molecular biological way for the rapid detection of C. burnetii.

RT-PCR and Western-blotting were used to detect the expression quantity of RAMP1.

Objective To discuss the clinical significance of detecting HBV-DNA by fluorescent PCR .

GlaxoSmithKline and Enigma Diagnostics have an agreement to develop point-of-care PCR tests to identify specific flu strains.

Conclusion 4-pair-primer-PCR is a quick, peculiar, sensitive method for the diagnosis and surveillance of plague.

In situ PCR amplification can be performed on fixed tissue or cells.

Intron length polymorphism (ILP) is a new type of PCR-based molecular markers with many advantages.

At present, PCR-CE has been widely used in microbiological detection.

In itself, a positive PCR is no barrier to getting a licence.

At present, PCR is applied to molecular biology, medicine, agronomy and other fields.

Conclusions The application of multiplex PCR on ABO genotyping has its unique features.

Identified by individual plant testing , analysis of RT-PCR and dot blot , this fragment was only existed in CMS cauliflower knxd612.

Conclusions: PCR is the more differential and sensitive method to detect H. pylori infection.

Compared with serologic tests, two PCR methods were evaluated in the diagnosis of syphilis.

PCR results showed that there were transgenes in the cotton genome.

Crime scene investigators routinely use the PCR technique to detect and identify vanishingly small amounts of DNA at crime scenes.

Conclusion FQ-PCR technique is valuble to confirmed the various forms lesion of early syphilis untreated.

Objective To establish PCR assay of Porcine Parvovirus detection in Trypsin.

P. C. R. has become a standard in monitoring an infected patient's response to antiretroviral therapy.

Objective To evaluate the value of PCR to pleural biopsy specimen in the diagnosis of tuberculous pleuritis .

The feasibility of TAIL-PCR in a soybean gene cloning was confirmed.

PCR and APX activity detection showed that transgenic plants were obtained.

ConclusionPCR methods can identify the genotype of the mice with SPARC gene mutant precisely.

In this system , we designed 2 independent injection pumps for sampling injection of blood sample and the PCR mixed solution separately .

Result The sensitivity of immuno-PCR was 300 times as sensitive as that of conventional ELISA.

Vodka paves the way for sustainable liquor packaging, with a 100% PCR PET bottle.

He calls the positive PCR a "potentially insurmountable hurdle" .

Mutants were identified by basic characteristic of Salmonella pullorum, special PCR and gene location.

Semi-quantitative RT-PCR analysis showed that ZF3 was expressed in root, stem, leaf, flower, immature pod and embryo of chickpea.

It is believed that RTQ-PCR method can detect the abundance of algicidal bacteria of Pseudomonas in environmental samples quantitatively.

Current multiplex polymerase chain reaction (PCR) techniques can at most offer detection from among 50 organisms in one test.

And set up the accurate andspeedly method of the duel real-time PCR on streptococcus suis serotype2 detection.

The NDI gene in mt DNA was amplified with regular PCR method, and the PCR product was sequenced after purified.

Methods To detect the peculiar gene fraction of Y. pestis in the Spermophilus dauricus viscera by PCR with internal comparison.

Here, we summarize our recent progress using directed RT-PCR cloning and DNA synthesis.

Utah-based DxNA has asked the U. S. Food and Drug Administration to approve its quick GeneSTAT PCR test.

and 5, carrying out PCR screening identification on the obtained primarily screened transgene plants.

The safety of biogenic amines metabolism of 22 fine Oenococcus omni (Oo) strains were studied with PCR and HPLC testing methods.

The products were transformed into BL21 competent cells, coated plates, select monoclonal colony PCR.

A multiplex- PCR method for detection bovine materials in foodstuffs.

PCR analyses showed that GFP DNA was not detectable in blood or distal tissues.

Therefore, determination of histopathology is necessary for definite diagnosis of liver TB supplemented with TB-PCR and AFB stain.

Methods Telomerase activity was analysed by PCR-ELISA technique.

Inspects all pre-assigned rooms for VIP's, returning guests and PCR members prior to arrival.

Conclusion Multiplex PCR is a high- flux, rapid method for clinical diagnosis of pathogens.

It is of broad interest to explore methods to solve these problems in PCR.

In recent years, with the developing and more detailed of the technology, RQ PCR has greatly improved the level of research on plant pests.

The results show that molecular identification based on PCR and direct sequencing is desirable for identifying thrips species.

Objective To establish a multiplex PCR assay for detecting Angiostrongylus cantonensis larvae in Pomacea canaliculata.

Objective To establish a rapid ABO genotyping method by using multiplex-PCR technique.

The optimizations of PCR and RFLP analysis conditions were studied.