METHODS Spectrophotometry and fluorescencequenchingmethod were used.
方法荧光猝灭法和紫外吸收光谱法。
2
Methods: Fluorescencequenchingmethod and Fster non-radiative energy transfer theory were used.
方法：主要应用了荧光猝灭法及能量转移原理。
3
The binding constant, the number of binding sites nand thermodynamic parameters were measured at different temperatures by fluorescencequenchingmethod.