The application of fluorescein dye and visualization under a cobalt blue light will reveal leaking aqueous humor, termed the Seidel test (shown).

The influence of gold nanoparticles on the optical properties of dye molecules of Rhodamine 6g (R6G) and fluorescein (fl) has been studied.

Fluorescein angiogram of these eyes showed there is no dye leakage from the retinal vessels.