Method TB protein-chips were used to detect serum samples of 63 TB patients, 25 non-TB patients and healthy subjects. The results were compared with acid-fast bacilli staining method.

Acid-fast staining was positive in all the 20 multibacillary leprosy cases, 18/30 paucibacillary leprosy cases, 7/9 other mycobacterial or fungal cases, negative in all the 10 non-infectious cases.

Results The positive rate of MTB detected with FQ-PCR was higher than that with smear acid-fast and culture of organism. The positive rate of non-tuberculosis was only 2.7%.