In this article the principle and methods in constructing RNA replicon-based vectors of Flavivirus virus their applications were reviewed.

West Nile virus (WNV) is a member of the flavivirus genus and belongs to the Japanese encephalitis antigenic complex of the family Flaviviridae.

Abstract: Like Yellow fever virus, Dengue virus, Japanese encephalitis virus and West Nile virus, Zika virus is also a mosquito-borne flavivirus.