Methods Immunohistochemistry and electrophoretic mobility shift assay (EMSA) were used.
方法采用免疫组化法及凝胶电泳迁移率实验。
2
First, we carried out Electrophoretic Mobility Shift Assay (EMSA) to investigate the binding difference of SNPs and transcription factors.
首先使用凝胶迁移检测和疾病关联的SNP是否会影响某些转录因子的结合。
3
The phosphorylation of STAT5 and its location were examined by double immunohistochemical staining and the binding of STAT5 with DNA was determined by electrophoretic mobility shift assay (EMSA).