Established upon the embryostem cell technique and homologous recombination, gene targeting has been widely used in the genome specific manipulation.
基因打靶技术是建立在胚胎干细胞和同源重组技术之上，可对基因组进行定点修饰的实验方法。
2
However, nuclear reprogramming, which stands in the way of reconstructed embryostem cell research, is still a bottleneck in the procedure of nuclear transfer.
但是核重编程仍是核移植技术的瓶颈，制约了重构胚胎干细胞的研究。
3
Scientists have found they can create chimeric animals that have organs belonging to another species by injecting stem cells into the embryo of another species.