MethodsChromatographic fingerprint of Semen Cassiae was investigated by RP-HPLC and the gradient elutionmode was applied to chromatographic separation.
方法采用RP - HPLC法,梯度洗脱,研究决明子饮片的指纹图谱,并作相似度比较分析。
2
Methods: The gradient elutionmode was applied in chromatographic separation and data were analyzed to develop HPLC fingerprint analysis of Safflor Yellow.
方法:采用梯度洗脱的方法进行色谱分离,对各色谱峰进行数据处理,得出红花黄色素的指纹图谱。
3
The elutionmode, composition of the mobile phase, content of the organic acid and the flow-rate of the mobile phase were optimized and thus a new flow rate gradient was proposed.